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  1. Link

    cd11b--cd11c-antibody-ox42-ab1211.pdf

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Neuroscience Cell Adhesion Proteins Receptors Integrins
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Anti-CD11b + CD11c antibody [OX42] (ab1211)

  • Datasheet
  • SDS
Reviews (6)Q&A (2)References (120)

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Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)
  • Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] (ab1211)
  • Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)

Key features and details

  • Mouse monoclonal [OX42] to CD11b + CD11c
  • Suitable for: IHC-Fr, ICC/IF
  • Reacts with: Rat
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Biotin

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Overview

  • Product name

    Anti-CD11b + CD11c antibody [OX42]
  • Description

    Mouse monoclonal [OX42] to CD11b + CD11c
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Rat
    Does not react with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.

  • Positive control

    • ICC/IF: NR8383 cells. IHC-Fr: Rat spleen and lung tissue sections (10µm).
  • General notes

    IHC protocol advice:

    This antibody is not suitable for IHC on paraffin-embedded samples.

    For best results in IHC on frozen tissue, the following may help detection:
    1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
    2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
    3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
    4) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
    5) Do not heat the samples or sections.
    6) During the staining procedure, do not allow the sections to dry out.

    Our Technical team (technical@abcam.com) will be happy to provide further information and advice.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b + CD11c equivalent antigen.
  • Clonality

    Monoclonal
  • Clone number

    OX42
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Receptors
    • Integrins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD11b + CD11c antibody [OX42] (ab216355)
    • Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524)
    • Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)
    • Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Related Products

    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (2)
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Application notes
Is unsuitable for IHC-P.

Target

  • Cellular localization

    CD11b: Membrane. CD11c: Membrane.
  • Database links

    • Entrez Gene: 25021 Rat
    • Entrez Gene: 499271 Rat

    Images

    • Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)
      Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)

      Immunofluorescence staining of CD11b + CD11c staining in a section of 10% formalin fixed (10 mins, RT) frozen rat spleen.

      Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab1211 at 1µg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1.5µg/ml) for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    • Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)
      Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of Carl Hobbs.

      ab1211 staining CD11b + CD11c (shown in red) in rat spleen tissue sections by IHC (PFA perfusion fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with BSA for 10 minutes at 21°C. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 16 hours. A Biotin-conjugated Goat anti mouse polyclonal (1/300) was used as the secondary antibody.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] (ab1211)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] (ab1211)

      ab1211 staining CD11b + CD11c in NR8383 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1211 at 1 µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 100% methanol (5 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)
      Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of an anonymous Abreview.

      ab1211 staining CD11b + CD11c (shown in green) in rat spleen tissue by IHC (Frozen sections). Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.

      See Abreview

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (120)

    Publishing research using ab1211? Please let us know so that we can cite the reference in this datasheet.

    ab1211 has been referenced in 120 publications.

    • Singh G  et al. Fetal inflammation induces acute immune tolerance in the neonatal rat hippocampus. J Neuroinflammation 18:69 (2021). PubMed: 33706765
    • Li Y  et al. Transcriptome profiling of long noncoding RNAs and mRNAs in spinal cord of a rat model of paclitaxel-induced peripheral neuropathy identifies potential mechanisms mediating neuroinflammation and pain. J Neuroinflammation 18:48 (2021). PubMed: 33602238
    • Yu S  et al. Activation of MC1R with BMS-470539 attenuates neuroinflammation via cAMP/PKA/Nurr1 pathway after neonatal hypoxic-ischemic brain injury in rats. J Neuroinflammation 18:26 (2021). PubMed: 33468172
    • Liu C  et al. Genistein-3'-sodium sulfonate Attenuates Neuroinflammation in Stroke Rats by Down-Regulating Microglial M1 Polarization through a7nAChR-NF-?B Signaling Pathway. Int J Biol Sci 17:1088-1100 (2021). PubMed: 33867831
    • Yao X  et al. Neuroprotective and Angiogenesis Effects of Levetiracetam Following Ischemic Stroke in Rats. Front Pharmacol 12:638209 (2021). PubMed: 34054520
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD11b + CD11c antibody [OX42]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Brandon Jun

    Verified customer

    Submitted Dec 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b + CD11c antibody [OX42]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Lung)
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted May 31 2013

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b + CD11c antibody [OX42]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Spleen)
    Specification
    Spleen
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 19 2011

    Immunohistochemistry free floating abreview for Anti-CD11b + CD11c antibody [OX42]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (liver)
    Specification
    liver
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 23 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b + CD11c antibody [OX42]

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Tissue sections (Microglia - cell culture)
    Permeabilization
    No
    Specification
    Microglia - cell culture
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 25 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b + CD11c antibody [OX42]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (adult liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization
    No
    Specification
    adult liver
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Oct 15 2008

    Question

    Is CD11b/c a good specific marker for microglia?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 13 2011

    Answer

    According to current literature, both CD11b and CD11c are suitable markers for microglia (neuronal macrophages).

    Please see below for a reference:
    http://www.jneuroinflammation.com/content/4/1/26

    However, please note that CD11b is also expressed in granulocytes, monocytes, NK cells, T cells, B cells and dendritic cells.

    CD11c is also expressed in monocytes, macrophages, granulocytes, T cell and B cell subsets.

    Please refer to our CD Antigen Chart and Microglia Markers page for more information:
    CD Antigen Chart Microglia Markers Read More

    Abcam Scientific Support

    Answered on Sep 13 2011

    Question

    I am curious if there is any new data concerning the cross reactivity of this antibody in mouse. I am also curious if there is a polyclonal form for mouse OX42. Please let me know. Thank you so much in advance for your reply.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    There has been no further information regarding this antibody cross reacting with mouse. OX42 is the clone name for ab1211 and only monoclonals have clone names, therefore it is impossible for there to be a polyclonal OX42. If you are after a polyclonal CD11b/c I am afraid we do not have this product.

    Read More

    Asdf Edo

    Abcam Scientific Support

    Answered on Oct 13 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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