Anti-CD11b + CD11c antibody [OX42] (ab1211)
![Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.com/ps/products/1/ab1211/Images/ab1211-273171-anti-cd11b-c-antibody-ox42-immunofluorescence.jpg "Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)")
Key features and details
- Mouse monoclonal [OX42] to CD11b + CD11c
- Suitable for: IHC-Fr, ICC/IF
- Reacts with: Rat
- Isotype: IgG2a
Overview
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Product name
Anti-CD11b + CD11c antibody [OX42] -
Description
Mouse monoclonal [OX42] to CD11b + CD11c -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RatIHC-Fr Rat
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Immunogen
Tissue, cells or virus corresponding to CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.
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Positive control
- ICC/IF: NR8383 cells, rat macrophages. IHC-Fr: Rat spleen and brain tissue.
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General notes
IHC protocol advice:
This antibody is not suitable for IHC on paraffin-embedded samples.
For best results in IHC on frozen tissue, the following may help detection:
1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
5) Do not heat the samples or sections.
6) During the staining procedure, do not allow the sections to dry out.Our Technical team (technical@abcam.com) will be happy to provide further information and advice.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b + CD11c equivalent antigen. -
Clonality
Monoclonal -
Clone number
OX42 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Rat
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| IHC-Fr |
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 5 - 10 µg/ml.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. |
Target
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Cellular localization
CD11b: Membrane. CD11c: Membrane. -
Database links
- Entrez Gene: 25021 Rat
- Entrez Gene: 499271 Rat
- Unigene: 54465 Rat
Images
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Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of Carl Hobbs.
ab1211 staining CD11b + CD11c (shown in red) in rat spleen tissue sections by IHC (PFA perfusion fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with BSA for 10 minutes at 21°C. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 16 hours. A Biotin-conjugated Goat anti mouse polyclonal (1/300) was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.com/ps/products/1/ab1211/Images/ab1211-266584-anti-cd11b-c-antibody-ox42-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD11b + CD11c antibody [OX42] (ab1211)NR8383 cells (rat macrophage cell line) stained for CD11b + CD11c (shown in green) using ab1211 in ICC/IF. The cells were fixed with 100% methanol for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3 M glycine in 0.1% Tween-PBS for 1 hour at room temperature to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1211 at 5µg/ml) overnight at +4°C. The secondary antibody was ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (shown in red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (shown in blue) at a concentration of 1.43 µM for 1 hour at room temperature.
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![Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.com/ps/products/1/ab1211/Images/ab1211-4083-anti-cd11b-c-antibody-ox42-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of an anonymous Abreview.ab1211 staining CD11b + CD11c (shown in green) in rat spleen tissue by IHC (Frozen sections). Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.
Protocols
References (96)
ab1211 has been referenced in 96 publications.
- Ding H et al. BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis. J Neuroinflammation 17:19 (2020). PubMed: 31931832
- Wang Y et al. Electroacupuncture Treatment Suppresses Transcription Factor IRF8 in Spinal Cord of Rats with Spared Nerve Injury. Pain Res Manag 2020:1854363 (2020). PubMed: 32351637
- Hu L et al. Microglia-Derived NLRP3 Activation Mediates the Pressor Effect of Prorenin in the Rostral Ventrolateral Medulla of Stress-Induced Hypertensive Rats. Neurosci Bull 36:475-492 (2020). PubMed: 32242284
- Rosas-Jarquín CJ et al. Chronic consumption of cassava juice induces cellular stress in rat substantia nigra. Iran J Basic Med Sci 23:93-101 (2020). PubMed: 32405352
- Chen R et al. Expression profiling of spinal cord dorsal horn in a rat model of complex regional pain syndrome type-I uncovers potential mechanisms mediating pain and neuroinflammation responses. J Neuroinflammation 17:162 (2020). PubMed: 32446302
