Recombinant Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)


  • Product name

    Anti-CD11b antibody [EP1345Y] - C-terminal
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [EP1345Y] to CD11b - C-terminal
  • Host species

  • Specificity

    Testing of mouse and rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
  • Tested applications

    Suitable for: ICC/IF, IHC-FoFr, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD11b aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11215

  • Positive control

    • IHC-P: Human spleen and human cervical cancer tissue WB: TF1 lysate. THP-1 macrophages, +10 ng/ml LPS. ICC/IF: THP-1 cell lysate IP: TF-1 whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-FoFr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 128 kDa.

For unpurified use at 1/20000 - 1/50000

IP 1/30.

For unpurified use at 1/80

IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1 - 5 µg/ml


  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3 antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue sections labeling CD11b with purified ab52478 at 1:1000 dilution (0.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Hematoxylin was used as a counterstain.

    Negative control: PBS instead of the primary antibody (inset).

  • Unpurified ab52478 staining CD11b in the THP-1 (Human monocytic leukemia cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI.

  • Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 0.3 µg/ml (purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 128 kDa

    Blocking and diluting buffer: 5% NFDM/TBST.

  • ab52478 (purified) at 1:30 dilution (2 µg) immunoprecipitating CD11b in TF-1 (Human bone marrow erythroleukemia cell line) whole cell lysate.
    Lane 1: TF-1 whole cell lysate 10 µg (input). 
    Lane 2: ab52478 + TF-1 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52478 in TF-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Enhanced expression of monocytes/ macrophage markers in the obese adipose tissue.

    The protein expression (intensity) of monocyte/ macrophage markers was detected by immunohistochemistry (IHC) in the adipose tissue samples from lean, overweight, and obese individuals, 10 each. As shown by representative IHC photomicrographs (100× magnification), expression of (A) CD11b was found to be markedly elevated in overweight and obese adipose tissue samples as compared with lean samples.

    Paraffin-embedded sections (4 μm thick) of subcutaneous adipose tissue were deparaffinized in xylene and rehydrated through descending grades of ethanol (100%, 95%, and 75%) to water. Antigen retrieval was performed under pressure cooker boiling for 8 min and cooling for 15 min. After washing in PBS, endogenous peroxidase activity was blocked with 3% H2O2 for 30 min and non-specific antibody binding was clocked with 5% nonfat milk for 1hr and 1% bovine serum albumin (BSA) solution for 1hr. Slides were treated overnight with primary antibodies at room temperature. After washing with PBS (0.5% Tween), slides were incubated for 1hr with secondary antibody conjugated with HRP polymer chain and color was developed using 3,3ʹ-diaminobenzidine chromogen substrate. Specimens were washed in running tap water, lightly counterstained with hematoxylin, dehydrated through ascending grades of ethanol (75%, 95%, and 100%), cleared in xylene, and finally mounted in dibutyl phthalate xylene (DPX).

  • Duchenne muscular dystrophy (DMD) muscle was co-stained for Neu5Gc (green), ab52478 (red) and DAPI (blue).

    For double immunostaining, sections were first stained overnight at 4°C with anti-Neu5Gc after blocking in 10% (Neu5Gc-free) human serum, after blocking in 5 mg/mL BSA, sections were incubated overnight with both primary antibodies without fixation, washed for one hour and incubated with the appropriate secondary antibodies.

  • IHC image of CD11b staining in a formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with unpurified ab52478, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 1/20000 dilution (unpurified) + TF-1 (Human bone marrow erythroleukemia cell line) lysate at 10 µg

    goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 128 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using unpurified ab52478 at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


This product has been referenced in:

  • Li D  et al. ß-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils. J Microbiol N/A:N/A (2019). Read more (PubMed: 30721460) »
  • Liu W & Rask-Andersen H Super-resolution immunohistochemistry study on CD4 and CD8 cells and the relation to macrophages in human cochlea. J Otol 14:1-5 (2019). Read more (PubMed: 30936894) »
See all 45 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (TUBO Breast Cancer Tumor)
Antigen retrieval step
Heat mediated
Yes - 0.1% Triton X-100
TUBO Breast Cancer Tumor
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Jun 22 2017

Western blot
Human Cell lysate - whole cell (THP-1)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Mar 27 2017

Immunohistochemistry (Frozen sections)
Human Tissue sections (human cells grown on interphase co-culture)
human cells grown on interphase co-culture
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted May 24 2016

Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain)
Antigen retrieval step
Yes - 3% Triton X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 08 2013

Western blot
Human Cell lysate - whole cell (THP-1 (human monocytic leukemia))
Loading amount
40 µg
THP-1 (human monocytic leukemia)
200 nM PMA for 72 hours (THP-1 macrophages)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Nov 07 2012


Product Anti-CD11b antibody [EP1345Y] (ab52478) is currently validated for use with human samples types, and is covered by our Abpromise Guarantee only for use with human sample types in the applications listed on the datasheet.

Testing of ab52478 with mouse and rat tissue samples (brain, spleen, kidney, and heart) in WB applications produced negative results. However, we did obtain positive results for use of this Ab in Flow Cytometry applications with the mouse RAW 264.7 cell line. Because of this variability in results when ab52478 is used with mouse samples, we do not at this time list mouse as a validated species for ab52478. Likewise, we do not recommend use of ab52478 with rat samples.

Additional information regarding our Abpromise Guarantee can be found here: Abpromise Guarantee. Read More


For Flow Cytometry experiments with ab52478 using RAW 246.7 cells were performed in a 100uL volume, using 1x10^6 cells.

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