Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 (human monocytic leukemia))
Loading amount
40 µg
Specification
THP-1 (human monocytic leukemia)
Treatment
200 nM PMA for 72 hours (THP-1 macrophages)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: LiCor Dk anti-Rb 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Detection
Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 180 kDa
Additional data
Additional Notes
Lane
1: THP-1 monocytes, untreated
2: THP-1 monocytes, +10 ng/ml LPS
3: THP-1 macrophages, untreated
4: THP-1 macrophages, +10 ng/ml LPS
Lysis buffer: RIPA +protease inhibitor cocktail
1: THP-1 monocytes, untreated
2: THP-1 monocytes, +10 ng/ml LPS
3: THP-1 macrophages, untreated
4: THP-1 macrophages, +10 ng/ml LPS
Lysis buffer: RIPA +protease inhibitor cocktail
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Nov 07 2012