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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant Anti-CD11b antibody [EPR1344] (ab133357)

  • Datasheet
  • SDS
Reviews (27) Submit a question References (119)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
  • Anti-CD11b antibody [EPR1344] (ab133357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1344] to CD11b
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CD11b antibody [EPR1344]
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [EPR1344] to CD11b
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD11b aa 1-100. The exact sequence is proprietary.
    Database link: P11215

  • Positive control

    • WB: THP1 cell lysate treated with TPA, and TF1 cell lysate; Rat spleen lysate IHC-P: Human tonsil and spleen tissues; Rat cerebrum and bone marrow tissue; Mouse lung and colon tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1344
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • HRP Anti-CD11b antibody [EPR1344] (ab204472)
    • Anti-CD11b antibody [EPR1344] - BSA and Azide free (ab209970)
    • Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (ab216445)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-TMEM119 antibody [28-3] - Microglial marker (ab209064)
    • Anti-CD68 antibody [FA-11] (ab53444)
    • Anti-CD11b antibody [M1/70] (ab8878)
    • Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Rat
Human
WB
Mouse
Rat
Human
All applications
Pig
Rhesus monkey
Application Abreviews Notes
WB (4)
1/1000. Predicted molecular weight: 127 kDa.
IHC-P (14)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please optimize IHC protocol when testing mouse and rat tissues. It is easy to show background staining in liver tissue.

Notes
WB
1/1000. Predicted molecular weight: 127 kDa.
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please optimize IHC protocol when testing mouse and rat tissues. It is easy to show background staining in liver tissue.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 25021 Rat
    • Entrez Gene: 714832 Rhesus monkey
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Unigene: 54465 Rat
    see all
  • Alternative names

    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3 antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)Min, M et al PLoS One. 2017 Dec 22;12(12):e0190051. doi: 10.1371/journal.pone.0190051. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Rottlerin decreases the number of effector cells that mainly infiltrate the skin in IMQ-treated mice

    Immunohistochemical detection of immune cell-related markers was performed on paraffin-embedded sections obtained from the back skin of IMQ-induced mice treated with vehicle or rottlerin.

    Representatives IHC images of CD11b (B) on the skin of the vehicle or rottlerin-treated mice. Scale bar = 100μm.

    Quantification analysis of IHC staining for CD11b(E) on the skin of the vehicle and rottlerin treated mice. Two independent researchers counted the number of positive staining cells were per high-power field (HPF). The data are representative of three experiments (n = 5 mice per group). ** P<0.01 vs. vehicle.

  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    All lanes : Anti-CD11b antibody [EPR1344] (ab133357) at 1/1000 dilution (purified)

    Lane 1 : TF-1 cell lysate
    Lane 2 : TPA treated THP-1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)This image is courtesy of an abreview from Anugraha Rajagopalan

    Formalin-fixed, 0.2% Triton-X permeabilized Mouse tissue sections (Eo771 breast cancer) was stained for CD11b using ab133357 (Green) at 1/1000 dilution in immunohistochemical analysis. The secondary antibody was a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) at 1/1000 dilution.

    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    Ab133357 staining CD11b in paraffin embedded Mouse lung tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.031 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells of mouse lung.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    Ab133357 staining CD11b in paraffin embedded Mouse colon tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.031 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells of mouse colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    Ab133357 staining CD11b in paraffin embedded Rat cerebrum tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/4000 dilution (0.29 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on gliocytes of rat cerebrum [PMID: 20483006].

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    Formaldehyde-fixed, paraffin-embedded rat bone marrow tissue stained for CD11b using ab133357 at 1/5000 in immunohistochemical analysis.

    Heat mediated antigen retrieval with EDTA buffer pH 9 was performed before commencing with staining protocol. 1% casein was used as blocking agent.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    IHC image of CD11b staining in a formalin fixed, paraffin embedded human normal spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab133357 at 1/4000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    Anti-CD11b antibody [EPR1344] (ab133357) at 1/1000 dilution + Rat spleen tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    Western blot - Anti-CD11b antibody [EPR1344] (ab133357)
    All lanes : purified at 1/10000 dilution

    Lane 1 : RAW264.7 cell lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 127 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] (ab133357)

    Immunohistochemical staining of paraffin embedded human spleen with purified ab133357 at a 1/4000 dilution. The secondary antibody used is a HRP goat anti-rabbit (ab97051). The sample is counterstained with hematoxylin.

    Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Anti-CD11b antibody [EPR1344] (ab133357)
    Anti-CD11b antibody [EPR1344] (ab133357)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (119)

    Publishing research using ab133357? Please let us know so that we can cite the reference in this datasheet.

    ab133357 has been referenced in 119 publications.

    • Huang Y  et al. A SIRPa-Fc fusion protein enhances the antitumor effect of oncolytic adenovirus against ovarian cancer. Mol Oncol 14:657-668 (2020). PubMed: 31899582
    • Zhang LY  et al. Microglia exacerbate white matter injury via complement C3/C3aR pathway after hypoperfusion. Theranostics 10:74-90 (2020). PubMed: 31903107
    • Li R  et al. Distinct effects of general anesthetics on lung metastasis mediated by IL-6/JAK/STAT3 pathway in mouse models. Nat Commun 11:642 (2020). PubMed: 32005799
    • Zhang K  et al. Silencing lncRNA Lfar1 alleviates the classical activation and pyoptosis of macrophage in hepatic fibrosis. Cell Death Dis 11:132 (2020). PubMed: 32071306
    • Shahulhameed S  et al. A Systematic Investigation on Complement Pathway Activation in Diabetic Retinopathy. Front Immunol 11:154 (2020). PubMed: 32117292
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 27 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Bone marrow)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    Bone marrow
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Dr. Queralt Vallmajó

    Verified customer

    Submitted Mar 18 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [EPR1344]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen)
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    Fish Gel as blocking agent for 40 minute(s) · Concentration: 4%
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 23 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 03 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD11b antibody [EPR1344]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Hippocampus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer, pH 6.0
    Permeabilization
    Yes - 0.1% Triton in PBS
    Specification
    Hippocampus
    Blocking step
    1% BSA + 5% Serum + 0.3 M Glycine as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Sergi Bayod

    Verified customer

    Submitted Nov 22 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [EPR1344]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (BRAIN)
    Permeabilization
    Yes - 1% Triton X-100
    Specification
    BRAIN
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 25 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [EPR1344]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    No
    Specification
    Heart
    Blocking step
    UV ultra-block (Thermo Fischer Scienti?c) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Acetone
    Read More

    Christopher Trummer

    Verified customer

    Submitted Sep 10 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 24 2018

    Western blot abreview for Anti-CD11b antibody [EPR1344]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Nerve)
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Loading amount
    15 µg
    Specification
    Nerve
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 29 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Eo771 breast cancer)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% Triton-X
    Specification
    Eo771 breast cancer
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Zinc fixative BD Pharmigen
    Read More

    Anugraha Rajagopalan

    Verified customer

    Submitted Mar 16 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
    Permeabilization
    Yes - H2O2
    Specification
    Skin (epidermis to SQ fat or muscle)
    Blocking step
    ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    10% neutral buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    1-10 of 27 Abreviews or Q&A

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