Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EPR1344]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Pig Tissue sections (Skin (epidermis to SQ fat or muscle))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Invitrogen eBioscience IHC antigen retrieval solution, low pH, 10x
Yes - H2O2
Skin (epidermis to SQ fat or muscle)
Blocking step
ab64226 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
10% neutral buffered formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight

Secondary antibody

Non-Abcam antibody was used: Abcam antibody was used but it would not let me su
Host species: Goat
Clonality: Polyclonal
Conjugation: Streptavidin

Additional data

Additional Notes
We tried the following dilutions: 1:500, 1:1000, 1:2000, 1:4000. Even when concentrated, there was no apparent staining.
Abcam response
Our Scientific Support team has investigated this negative Abreview, and the issue has been resolved in accordance with our Abpromise.

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Submitted Feb 28 2018

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