Brain fixed in 4% PFA (in PBS) at 4ºC for 24 h.
Heat-induced antigen retrieval with Citrate buffer (10 mM Citric acid, 0.05% Tween 20, pH 6.0), at 80ºC for 15 min, using a water bath.
Image: CD11b ab133357 (green) and Hoechst (blue).
Counterstained with Hoechst, 2 ug/ml for 5 min at room temperature.
Mounted in Fluoromount-G.
Dr. Sergi Bayod
Submitted Nov 22 2018
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