Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD11b antibody [EPR1344]

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate buffer, pH 6.0
Permeabilization
Yes - 0.1% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum + 0.3 M Glycine as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% Triton in PBS with 1% BSA
Dilution
1/200

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Brain fixed in 4% PFA (in PBS) at 4ºC for 24 h. Heat-induced antigen retrieval with Citrate buffer (10 mM Citric acid, 0.05% Tween 20, pH 6.0), at 80ºC for 15 min, using a water bath. Image: CD11b ab133357 (green) and Hoechst (blue). Counterstained with Hoechst, 2 ug/ml for 5 min at room temperature. Mounted in Fluoromount-G.

Dr. Sergi Bayod

Verified customer

Submitted Nov 22 2018

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