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  1. Link

    cd11b-antibody-epr19373-ab184307.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant Anti-CD11b antibody [EPR19373] (ab184307)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)
  • Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)
  • Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
  • Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
  • Anti-CD11b antibody [EPR19373] (ab184307)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19373] to CD11b
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
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Anti-FRMPD4 antibody (ab113420)
Protein
Recombinant Human CD11b protein (ab126010)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CD11b antibody [EPR19373]
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [EPR19373] to CD11b
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat spleen lysates; C6, M1, RAW 264.7 and J774A.1 whole cell lysates. Flow Cyt: RAW 264.7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19373
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-CD11b antibody [EPR19373] - BSA and Azide free (ab250744)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/120.
WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).
Notes
Flow Cyt
1/120.
WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 25021 Rat
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)
      Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)

      Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukaemia virus) cell line labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-  Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

    • Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)
      Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)

      Flow cytometric analysis of 4% paraformaldehyde-fixed WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) cells labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

      Negative control: WEHI-231 is negative for CD11b.

    • Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
      Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
      Lanes 1-3 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
      Lanes 4-5 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution

      Lane 1 : Mouse spleen lysate
      Lane 2 : M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate
      Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
      Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
      Lane 5 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 127 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).

    • Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
      Western blot - Anti-CD11b antibody [EPR19373] (ab184307)
      All lanes : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution

      Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
      Lane 2 : Rat spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 127 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370).

    • Anti-CD11b antibody [EPR19373] (ab184307)
      Anti-CD11b antibody [EPR19373] (ab184307)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab184307? Please let us know so that we can cite the reference in this datasheet.

    ab184307 has not yet been referenced specifically in any publications.

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