Recombinant Anti-CD11b antibody [EPR19373] (ab184307)
Overview
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Product name
Anti-CD11b antibody [EPR19373]
See all CD11b primary antibodies -
Description
Rabbit monoclonal [EPR19373] to CD11b -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse CD11b aa 150-350. The exact sequence is proprietary.
Database link: P05555 -
Positive control
- WB: Mouse and rat spleen lysates; C6, M1, RAW 264.7 and J774A.1 whole cell lysates. Flow Cyt: RAW 264.7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19373 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab184307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/120. | |
| WB | 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16409 Mouse
- Entrez Gene: 25021 Rat
- SwissProt: P05555 Mouse
- Unigene: 262106 Mouse
- Unigene: 54465 Rat
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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![Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.com/ps/products/184/ab184307/Images/ab184307-255700-anti-cd11b-antibody-epr19373-flow-cytometry.jpg)
Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)Flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukaemia virus) cell line labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]- Isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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![Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.com/ps/products/184/ab184307/Images/ab184307-255699-anti-cd11b-antibody-epr19373-flow-cytometry.jpg)
Flow Cytometry - Anti-CD11b antibody [EPR19373] (ab184307)Flow cytometric analysis of 4% paraformaldehyde-fixed WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) cells labeling CD11b with ab184307 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Negative control: WEHI-231 is negative for CD11b.
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![Western blot - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.com/ps/products/184/ab184307/Images/ab184307-255702-anti-cd11b-antibody-epr19373-western-blot.jpg)
Western blot - Anti-CD11b antibody [EPR19373] (ab184307)Lanes 1-3 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lanes 4-5 : Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).
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![Western blot - Anti-CD11b antibody [EPR19373] (ab184307)](https://www.abcam.com/ps/products/184/ab184307/Images/ab184307-255701-anti-cd11b-antibody-epr19373-western-blot.jpg)
Western blot - Anti-CD11b antibody [EPR19373] (ab184307)All lanes : Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370).
Datasheets and documents
Certificate of Compliance
References
ab184307 has not yet been referenced specifically in any publications.
