Recombinant Anti-CD11b antibody [EPR19387] (ab184308)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19387] to CD11b
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD11b antibody [EPR19387]
See all CD11b primary antibodies -
Description
Rabbit monoclonal [EPR19387] to CD11b -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: J774A.1, M1 and RAW 264.7 whole cell lysates. ICC/IF: RAW 264.7 cells. Flow Cyt: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19387 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa).
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ICC/IF | (1) |
1/500.
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IP |
1/40.
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Flow Cyt |
1/70.
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Notes |
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WB
1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 127 kDa). |
ICC/IF
1/500. |
IP
1/40. |
Flow Cyt
1/70. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Flow cytometric analysis of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling CD11b with ab184308 at 1/70 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-CD11b antibody [EPR19387] (ab184308) at 1/1000 dilution
Lane 1 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 2 : M1 (Mouse myeloblast myeloid leukemia cell line) whole cell lysate
Lane 3 : WEHI-231 (Mouse B cell lymphoma cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID: 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID: 2457584).
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Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) and WEHI-231 (Mouse B cell lymphoma cell line) cells labeling CD11b with ab184308 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1,000 dilution (green).
Confocal image showing membrane staining on RAW 264.7 cells and no staining on WEHI-231 cells (negative cell line).
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1,000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1,000 dilution (red).
The negative controls are as follows:
-ve control 1: ab184308 at 1/500 dilution followed by ab150120 at 1/1,000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1,000 dilution. -
CD11b was immunoprecipitated from 0.35 mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab184308 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184308 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).
Lane 2: ab184308 IP in RAW 264.7 whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab184308 in RAW 264.7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (12)
ab184308 has been referenced in 12 publications.
- Jiang H et al. M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway. Cell Death Dis 13:183 (2022). PubMed: 35210436
- Li Y et al. Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice. Front Pharmacol 13:850187 (2022). PubMed: 35370707
- Zhang X & Dong S Protective effect of growth differentiation factor 15 in sepsis by regulating macrophage polarization and its mechanism. Bioengineered 13:9687-9707 (2022). PubMed: 35420978
- Li Q et al. Therapy of spinal cord injury by folic acid polyethylene glycol amine-modified zeolitic imidazole framework-8 nanoparticles targeted activated M/Ms. Front Bioeng Biotechnol 10:959324 (2022). PubMed: 36185443
- Chen Y et al. Cocktail of isobavachalcone and curcumin enhance eradication of Staphylococcus aureus biofilm from orthopedic implants by gentamicin and alleviate inflammatory osteolysis. Front Microbiol 13:958132 (2022). PubMed: 36212814