Anti-CD11b antibody [M1/70] (ab8878)
Key features and details
- Rat monoclonal [M1/70] to CD11b
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG2b
Overview
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Product name
Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF Mouse -
Immunogen
corresponding to CD11b.
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Positive control
- Flow Cytometry: Human macrophages and monocytes; IF/ICC: RAW 264.7 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
M1/70 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- PerCP Anti-CD11b antibody [M1/70] (ab237025)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Human
|
ICC/IF |
Mouse
|
All applications |
Rabbit
|
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (4) |
Use a concentration of 1 - 5 µg/ml.
|
Flow Cyt | (2) |
Use 0.1-1µg for 106 cells.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
Flow Cyt
Use 0.1-1µg for 106 cells. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Entrez Gene: 100351865 Rabbit
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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ab8878 stained in Raw264.7 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8878 at 1µg/ml overnight at +4°C. The secondary antibodies was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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Overlay histogram showing Human monocytes stained with ab8878 (red line). PBMCs were pre-incubated in Human Fc block for 30 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC.
Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 band pass filter.
Human monocytes were identified with CD14 APC (ab91144) multi-color staining.
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Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Protocols
References (92)
ab8878 has been referenced in 92 publications.
- Zhou J et al. LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3. J Neuroinflammation 17:134 (2020). PubMed: 32345320
- Yue X et al. Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats. Neuropsychiatr Dis Treat 16:891-900 (2020). PubMed: 32308392
- Jaberi E et al. Identification of unique and shared mitochondrial DNA mutations in neurodegeneration and cancer by single-cell mitochondrial DNA structural variation sequencing (MitoSV-seq). EBioMedicine 57:102868 (2020). PubMed: 32629384
- Locke M et al. Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci Rep 10:11694 (2020). PubMed: 32678135
- Taylor X et al. A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy. J Neuroinflammation 17:223 (2020). PubMed: 32711525