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    cd11b-antibody-m170-ab8878.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Anti-CD11b antibody [M1/70] (ab8878)

  • Datasheet
  • SDS
Reviews (17)Q&A (1)References (92)

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
  • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
  • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

Key features and details

  • Rat monoclonal [M1/70] to CD11b
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

You may also be interested in

Protein
Recombinant Human CD11b protein (ab126010)
Primary
Product image
Anti-CD11b antibody [EPR19387] (ab184308)
Isotype
Rat IgG2b, kappa monoclonal [RTK4530] - Isotype Control - BSA and Azide free (ab18536)

View more associated products

Overview

  • Product name

    Anti-CD11b antibody [M1/70]
    See all CD11b primary antibodies
  • Description

    Rat monoclonal [M1/70] to CD11b
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    See all applications and species data
  • Immunogen

    corresponding to CD11b.

  • Positive control

    • Flow Cytometry: Human macrophages and monocytes; IF/ICC: RAW 264.7 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    M1/70
  • Myeloma

    NS1
  • Isotype

    IgG2b
  • Light chain type

    unknown
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
    • Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
    • Alexa Fluor® 405 Anti-CD11b antibody [M1/70] (ab197703)
    • PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
    • PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
    • PerCP Anti-CD11b antibody [M1/70] (ab237025)
    • violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
    • FITC Anti-CD11b antibody [M1/70] (ab24874)
    • PE Anti-CD11b antibody [M1/70] (ab25175)
    • redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
    • APC Anti-CD11b antibody [M1/70] (ab25482)
    • PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    • APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
All applications
Rabbit
Application Abreviews Notes
ICC/IF (4)
Use a concentration of 1 - 5 µg/ml.
Flow Cyt (2)
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Flow Cyt
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 100351865 Rabbit
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)

      ab8878 stained in Raw264.7 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8878 at 1µg/ml overnight at +4°C. The secondary antibodies was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
      Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

      Overlay histogram showing Human monocytes stained with ab8878 (red line). PBMCs were pre-incubated in Human Fc block for 30 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC.

      Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 band pass filter.

      Human monocytes were identified with CD14 APC (ab91144) multi-color staining.

    • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
      Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

      Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (92)

    Publishing research using ab8878? Please let us know so that we can cite the reference in this datasheet.

    ab8878 has been referenced in 92 publications.

    • Zhou J  et al. LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3. J Neuroinflammation 17:134 (2020). PubMed: 32345320
    • Yue X  et al. Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats. Neuropsychiatr Dis Treat 16:891-900 (2020). PubMed: 32308392
    • Jaberi E  et al. Identification of unique and shared mitochondrial DNA mutations in neurodegeneration and cancer by single-cell mitochondrial DNA structural variation sequencing (MitoSV-seq). EBioMedicine 57:102868 (2020). PubMed: 32629384
    • Locke M  et al. Fibrinogen protects neutrophils from the cytotoxic effects of histones and delays neutrophil extracellular trap formation induced by ionomycin. Sci Rep 10:11694 (2020). PubMed: 32678135
    • Taylor X  et al. A1 reactive astrocytes and a loss of TREM2 are associated with an early stage of pathology in a mouse model of cerebral amyloid angiopathy. J Neuroinflammation 17:223 (2020). PubMed: 32711525
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 18 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [M1/70]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (eye)
    Permeabilization
    No
    Specification
    eye
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 12 2020

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (C20 human microglia cell line)
    Permeabilization
    No
    Gating Strategy
    Microglia cells, CD11b positive
    Specification
    C20 human microglia cell line
    Preparation
    Cell harvesting/tissue preparation method: Microglia cells were harvested after cell dissociation by 5mM EDTA
    Sample buffer: PBS/BSA 2%
    Fixation
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 26 2018

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Mouse adult brain)
    Permeabilization
    No
    Gating Strategy
    Microglia cells, CD11b positive
    Specification
    Mouse adult brain
    Preparation
    Cell harvesting/tissue preparation method: Brain cells (microglia) were isolated using Adult Brain Dissociation Kit and gentleMACS™ Dissociator, both from Miltenylbiotec
    Sample buffer: PBS/BSA 2%
    Fixation
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 05 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human primary immortalised microglia cells)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    Human primary immortalised microglia cells
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 05 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [M1/70]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7
    Permeabilization
    Yes - Methanol/H2O2
    Specification
    Brain
    Blocking step
    A/B&DAKOprotein block & 2% bovine serum as blocking agent for 40 minute(s) · Concentration: 0% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 06 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [M1/70]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (synovium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid
    Permeabilization
    No
    Specification
    synovium
    Blocking step
    SuperBlock™ (PBS) Blocking Buffer as blocking agent for 15 minute(s) · Concentration: 0% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse primary microglia cells)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    mouse primary microglia cells
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Oct 10 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b [M1/70] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b [M1/70] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Mouse Tissue sections (whole mouse embryo)
    Specification
    whole mouse embryo
    Permeabilization
    Yes - 0.1% triton x100 in TBS
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 31 2013

    Immunocytochemistry abreview for Anti-CD11b [M1/70] antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (RAW264.7 cell line)
    Specification
    RAW264.7 cell line
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    1-10 of 18 Abreviews or Q&A

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