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  1. Link

    cd11b-antibody-m170-ab8878.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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Recombinant

Recombinant Anti-CD11b antibody [M1/70] (ab8878)

  • Datasheet
  • SDS
Reviews (17)Q&A (1)References (115)

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
  • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
  • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [M1/70] to CD11b
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC APC/Cy7® FITC PE PE/Cy5® PE/Cy7® PerCP/Cy5.5® redFluor™ 710 violetFluor™ 450

You may also be interested in

Protein
Recombinant Human CD11b protein (ab126010)
Primary
Product image
Anti-CD11b antibody [EPR19387] (ab184308)
Primary
Product image
PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)

View more associated products

Overview

  • Product name

    Anti-CD11b antibody [M1/70]
    See all CD11b primary antibodies
  • Description

    Rat monoclonal [M1/70] to CD11b
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit
  • Immunogen

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cytometry: Human macrophages and monocytes; IF/ICC: Raw264.7 (LPS treated) cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    M1/70
  • Myeloma

    NS1
  • Isotype

    IgG2b
  • Light chain type

    unknown
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
    • Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
    • PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
    • PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
    • violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
    • FITC Anti-CD11b antibody [M1/70] (ab24874)
    • PE Anti-CD11b antibody [M1/70] (ab25175)
    • redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
    • APC Anti-CD11b antibody [M1/70] (ab25482)
    • PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
    • APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (4)
Use a concentration of 1 - 5 µg/ml.
Flow Cyt (2)
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
Flow Cyt
Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Entrez Gene: 16409 Mouse
    • Entrez Gene: 100351865 Rabbit
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • SwissProt: P05555 Mouse
    • Unigene: 172631 Human
    • Unigene: 262106 Mouse
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)

      ab8878 staining CD11b in Raw264.7 (LPS treated) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab8878 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
      Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

      Overlay histogram showing Human monocytes stained with ab8878 (red line). PBMCs were pre-incubated in Human Fc block for 30 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC.

      Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 band pass filter.

      Human monocytes were identified with CD14 APC (ab91144) multi-color staining.

    • Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
      Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)

      Overlay histogram showing Human macrophages stained with ab8878 (red line). Cells were pre-incubated in Human AB serum (10%) for 20 mins at 4ºC. Cells were then incubated with the antibody (ab8878, 0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was a goat anti-rat Alexa Fluor® 488 (IgG H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (115)

    Publishing research using ab8878? Please let us know so that we can cite the reference in this datasheet.

    ab8878 has been referenced in 115 publications.

    • Gao R  et al. Macrophage-derived netrin-1 drives adrenergic nerve-associated lung fibrosis. J Clin Invest 131:N/A (2021). PubMed: 33393489
    • Yeh SI  et al. Pigment Epithelium-Derived Factor Peptide Promotes Corneal Nerve Regeneration: An In Vivo and In Vitro Study. Invest Ophthalmol Vis Sci 62:23 (2021). PubMed: 33481984
    • Yeini E  et al. P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression. Nat Commun 12:1912 (2021). PubMed: 33771989
    • Bain JM  et al. Immune cells fold and damage fungal hyphae. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33876755
    • Kim J  et al. Sorafenib Modulates the LPS- and Aß-Induced Neuroinflammatory Response in Cells, Wild-Type Mice, and 5xFAD Mice. Front Immunol 12:684344 (2021). PubMed: 34122447
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 18 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [M1/70]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (eye)
    Permeabilization
    No
    Specification
    eye
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 12 2020

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (C20 human microglia cell line)
    Permeabilization
    No
    Gating Strategy
    Microglia cells, CD11b positive
    Specification
    C20 human microglia cell line
    Preparation
    Cell harvesting/tissue preparation method: Microglia cells were harvested after cell dissociation by 5mM EDTA
    Sample buffer: PBS/BSA 2%
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Nov 26 2018

    Flow Cytometry abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Mouse adult brain)
    Permeabilization
    No
    Gating Strategy
    Microglia cells, CD11b positive
    Specification
    Mouse adult brain
    Preparation
    Cell harvesting/tissue preparation method: Brain cells (microglia) were isolated using Adult Brain Dissociation Kit and gentleMACS™ Dissociator, both from Miltenylbiotec
    Sample buffer: PBS/BSA 2%
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Nov 05 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human primary immortalised microglia cells)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    Human primary immortalised microglia cells
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Nov 05 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [M1/70]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH7
    Permeabilization
    Yes - Methanol/H2O2
    Specification
    Brain
    Blocking step
    A/B&DAKOprotein block & 2% bovine serum as blocking agent for 40 minute(s) · Concentration: 0% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 06 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [M1/70]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (synovium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid
    Permeabilization
    No
    Specification
    synovium
    Blocking step
    SuperBlock™ (PBS) Blocking Buffer as blocking agent for 15 minute(s) · Concentration: 0% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11b antibody [M1/70]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse primary microglia cells)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    mouse primary microglia cells
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    Submitted Oct 10 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b [M1/70] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b [M1/70] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: RT°C
    Sample
    Mouse Tissue sections (whole mouse embryo)
    Specification
    whole mouse embryo
    Permeabilization
    Yes - 0.1% triton x100 in TBS
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 31 2013

    Immunocytochemistry abreview for Anti-CD11b [M1/70] antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (RAW264.7 cell line)
    Specification
    RAW264.7 cell line
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    1-10 of 18 Abreviews or Q&A

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