Overview

  • Product name

    Anti-CD11b antibody [M1/70] (FITC)
    See all CD11b primary antibodies
  • Description

    Rat monoclonal [M1/70] to CD11b (FITC)
  • Host species

    Rat
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit
  • Immunogen

    Tissue/ cell preparation (Mouse): C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes.

  • Positive control

    • BALB/c spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab24874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab136125 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3 antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Images

  • Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab24874 at 0.5µg (solid line), Rat IgG2b (FITC)isotype control (dashed line) at 0.5µg concentration.

  • ab24874 at 1 µg/106 cells staining BALB/c splenocytes. Cells were then double stained with Rat monoclonal [RB6-8C5] to Ly6g (Phycoerythrin) (ab24884).  Lymphocytes were gated and analyzed by flow cytometry.

References

This product has been referenced in:

  • Kawano T  et al. Temporal and spatial profile of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) in ischemic stroke in mice. PLoS One 14:e0215482 (2019). Read more (PubMed: 31048856) »
  • Karlen SJ  et al. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration. J Neuroinflammation 15:344 (2018). Read more (PubMed: 30553275) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100 in TBS
Specification
mouse hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 10 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (liver)
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
OCT

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: cell suspension
Sample buffer: PBS
Permeabilization
No
Gating Strategy
Live cells

Abcam user community

Verified customer

Submitted May 19 2010

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