Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CD11b antibody [MEM-174] (Phycoerythrin)
See all CD11b primary antibodies -
DescriptionMouse monoclonal [MEM-174] to CD11b (Phycoerythrin)
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Host speciesMouse
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ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
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Tested applicationsSuitable for: Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Human granulocytes
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General notes
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
PurityIgG fraction
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Purification notesSize exclusion chromatography.
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ClonalityMonoclonal
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Clone numberMEM-174
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab28101 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 20µl for 106 cells. Use 20µl for 106 cells or 100µl of whole blood. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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FunctionIntegrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
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Tissue specificityPredominantly expressed in monocytes and granulocytes.
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Involvement in diseaseGenetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
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Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Omim: 120980 Human
- SwissProt: P11215 Human
- Unigene: 172631 Human
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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![Flow Cytometry - Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)](https://www.abcam.com/ps/products/28/ab28101/Images/ab28101-284944-ab28101fc.jpg)
Flow Cytometry - Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)Flow cytometry analysis of human peripheral blood cells labelling CD11b with ab28101.
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![Flow Cytometry - Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)](https://www.abcam.com/ps/products/28/ab28101/Images/ab28101-207014-ab28101fc.jpg)
Flow Cytometry - Anti-CD11b antibody [MEM-174] (Phycoerythrin) (ab28101)This image is courtesy of an Abreview submitted by Ina Laura Pieperab28101 staining CD11b in Human peripheral blood cells by Flow Cytometry. The sample was incubated with the primary antibody (1/6) for 30 minutes at 4°C. Gating Strategy: Granulocytes.
Protocols
References
ab28101 has not yet been referenced specifically in any publications.
