Overview

  • Product name

    Anti-CD11b antibody [SP330] - C-terminal
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [SP330] to CD11b - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD11b aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11215

  • Positive control

    • WB: TF-1 cell lysate. IHC-P: Human tonsil, colon, spleen, bone marrow, lung, cerebellum, thymus, lymph node, HK lymphoma, pancreatic adenocarcinoma and endometrial adenocarcinoma tissues. Flow Cyt: TF-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab224805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Primary incubation for 30 minutes at at 4°C.

WB 1/400. Predicted molecular weight: 127 kDa.

Primary incubation for 1 hour at room temperature.

IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen CD11b (p170) antibody
    • Antigen CD11b p170 antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11b/CD18 antibody
    • CD49d antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • Complement component 3 receptor 3 subunit antibody
    • Complement Component Receptor 3 Alpha antibody
    • Complement receptor type 3 antibody
    • Complement receptor type 3, alpha subunit antibody
    • CR 3 alpha chain (CR3A) antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3 antibody
    • CR3A antibody
    • F730045J24Rik antibody
    • Integrin Alpha M antibody
    • Integrin alpha M chain antibody
    • Integrin alpha-M antibody
    • Integrin beta 2 alpha subunit antibody
    • Integrin subunit alpha M antibody
    • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Ly-40 antibody
    • MAC 1 antibody
    • Mac-1a antibody
    • MAC1 antibody
    • Mac1, alpha subunit antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • Mo1 antibody
    • Mo1, alpha subunit antibody
    • MO1A antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • SLEB6 antibody
    see all

Images

  • Flow cytometric analysis of TF-1 (human bone marrow erythroleukemia cell line) cell line labeling CD11b with ab224805 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue) .

  • Anti-CD11b antibody [SP330] - C-terminal (ab224805) at 1/100 dilution + TF-1 (human bone marrow erythroleukemia cell line) cell lysate

    Predicted band size: 127 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cerebellum tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

References

ab224805 has not yet been referenced specifically in any publications.

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