• Product name
    Anti-CD11b/c antibody [OX42] (Biotin)
    See all CD11b/c primary antibodies
  • Description
    Mouse monoclonal [OX42] to CD11b/c (Biotin)
  • Host species
  • Conjugation
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat peritoneal macrophages

  • Positive control
    • Rat peritoneal macrophages
  • General notes

    ab33827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.



Our Abpromise guarantee covers the use of ab33827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.





ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Samples need to be PLP (periodate-lysine-paraformaldehyde) fixed.

ICC/IF Use at an assay dependent concentration.


  • Function
    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity
    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease
    Systemic lupus erythematosus 6
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD11b (p170) antibody
    • CD11 antigen like family member B antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member B antibody
    • CD11b antibody
    • CD11C antibody
    • Cell surface glycoprotein MAC 1 subunit alpha antibody
    • Cell surface glycoprotein MAC-1 subunit alpha antibody
    • CR 3 alpha chain antibody
    • CR-3 alpha chain antibody
    • CR3A antibody
    • Integrin alpha M antibody
    • Integrin alpha X antibody
    • Integrin alpha-M antibody
    • Integrin, alpha M (complement component 3 receptor 3 subunit) antibody
    • Integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • Integrin, alpha X (complement component 3 receptor 4 subunit) antibody
    • ITAM_HUMAN antibody
    • ITGAM antibody
    • ITGAX antibody
    • Leu M5, alpha subunit antibody
    • Leukocyte adhesion glycoprotein p150,95 alpha chain antibody
    • Leukocyte adhesion receptor MO1 antibody
    • Leukocyte adhesion receptor p150,95 antibody
    • Leukocyte surface antigen p150,95, alpha subunit antibody
    • MAC 1 antibody
    • MAC1A antibody
    • Macrophage antigen alpha polypeptide antibody
    • MGC117044 antibody
    • MO1A antibody
    • Myeloid membrane antigen, alpha subunit antibody
    • Neutrophil adherence receptor alpha M subunit antibody
    • Neutrophil adherence receptor antibody
    • OTTHUMP00000163299 antibody
    • p150 95 integrin alpha chain antibody
    • SLEB6 antibody
    see all


  • FACS analysis of rat peritoneal macrophages using ab33827.


This product has been referenced in:
  • Xie Y  et al. Electro-acupuncture stimulation prevents remifentanil-induced postoperative hyperalgesia by suppressing spinal microglia in rats. Exp Ther Med 16:353-359 (2018). Read more (PubMed: 29896261) »
  • Tsuruta T  et al. Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia. PLoS One 13:e0208938 (2018). Read more (PubMed: 30533035) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Yes, a biotin-conjugated Ab can be readily detected via streptavidin conjugated to HRP. The biotin-streptavidin interaction is very specific and of very high affinity.

One option for a ready-to-use streptavidin-peroxidase product can be found here: Streptavidin Peroxidase (Ready to Use). Read More


The following references report use of Ab clone OX42 in fluorescent staining applications:
Stidworthy MF, et al. Notch1 and Jagged1 are expressed after CNS demyelination, but are not a major rate-determining factor during remyelination. Brain. 2004 Sep;127(Pt 9):1928-41. Epub 2004 Aug 2. PMID: 15289265.

We would generally recommend acetone fixation for frozen sections, as used in the publication below:
Lehrmann E, et al. Microglial and macrophage reactions mark progressive changes and define the penumbra in the rat neocortex and striatum after transient middle cerebral artery occlusion. J Comp Neurol. 1997 Sep 29;386(3):461-76. PMID: 9303429.

The reference by Milligan et al. would suggest that paraformaldehyde fixation is not suitable for this antibody; however, please note that they used PLP fixed tissues successfully:
Milligan CE, et al. Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain. J Comp Neurol. 1991 Dec 1;314(1):125-35. PMID: 1797868.

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