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Synthetic peptide within Human CD11c aa 1125-1154 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P20702
Our Abpromise guarantee covers the use of ab175888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 127 kDa.|
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human hepatocarcinoma tissue labeling CD11c with ab175888 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunofluorescent analysis of Human hepatocarcinoma tissue labeling CD11c with ab175888 at 0.05 mg/ml followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
Flow Cytometric analysis of SK-Br-3 cells labeling CD11c with ab175888 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175888 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"