Recombinant Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1347Y] to CD11c - C-terminal
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD11c antibody [EP1347Y] - C-terminal
See all CD11c primary antibodies -
Description
Rabbit monoclonal [EP1347Y] to CD11c - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate; THP-1 + TPA lysate. IHC-P: Human tonsil, lymph node and spleen tissues. IP: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1347Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- HRP Anti-CD11c antibody [EP1347Y] (ab195534)
- Anti-CD11c antibody [EP1347Y] - BSA and Azide free (ab216655)
- Alexa Fluor® 647 Anti-CD11c antibody [EP1347Y] (ab275338)
- Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y] (ab275738)
- Alexa Fluor® 594 Anti-CD11c antibody [EP1347Y] (ab277235)
- Alexa Fluor® 555 Anti-CD11c antibody [EP1347Y] (ab279329)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (12) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. |
WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
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IP |
1/20.
For unpurified use at 1/80. |
Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. |
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa). |
IP
1/20. For unpurified use at 1/80. |
Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3687 Human
- Omim: 151510 Human
- SwissProt: P20702 Human
- Unigene: 248472 Human
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma sections labeling CD11c with ab52632 at 1:5000 dilution (0.02 μg/ml). Heat mediated antigen retrieval was performed using Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) (ready to use) secondary antibody was used. Positive staining on human Hodgkin's lymphoma. The section was incubated with ab52632 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD11c with ab52632 at 1:5000 dilution (0.02 μg/ml). Heat mediated antigen retrieval was performed using Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) (ready to use) secondary antibody was used. Positive staining on human spleen. The section was incubated with ab52632 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD11c with Purified ab52632 at 1:500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (purified)
Lane 1 : Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysates
Lane 2 : THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 128 kDaBlocking and diluting buffer: 5% NFDM/TBST
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ab52632 (purified) at 1:20 dilution (2µg) immunoprecipitating CD11c in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate 10ug
Lane 2 (+): ab52632 & THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52632 in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST." -
Immunohsitochemical analysis of paraffin-embedded human tonsil tissue labelling CD11c with ab52632 at 1/500 dilution. Tissue fixed with 10% NBF. An Alexa Fluor® 647 Goat anti-Rabbit IgG secondary antibody was used. Heat mediated antigen retrieval with Tris/EDTA, ph 9 carried out.
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Unpurified ab52632 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with ab52632 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.
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All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified)
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52632 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified) + THP-1 + TPA lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 128 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Unpurified ab52632 showing negative staining in Normal breast tissue.
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Unpurified ab52632 showing negative staining in Skeletal muscle tissue.
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Tissue Microarrays stained for "Anti-CD11c antibody [EP1347Y] - C-terminal” using "ab52632" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab52632 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (99)
ab52632 has been referenced in 99 publications.
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- Sona C et al. Evidence of islet CADM1-mediated immune cell interactions during human type 1 diabetes. JCI Insight 7:N/A (2022). PubMed: 35133983
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Chang ZY et al. Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice. Int J Mol Sci 23:N/A (2022). PubMed: 35269806
- Yang J et al. Dynamic profiling of immune microenvironment during pancreatic cancer development suggests early intervention and combination strategy of immunotherapy. EBioMedicine 78:103958 (2022). PubMed: 35316682