Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lymph node with melanoma metastasis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
Yes - Tween
Specification
lymph node with melanoma metastasis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s)
Dilution
1/100
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
This antibody was stained and imaged for Multiplexed Ion Beam Imaging by Time Of Flight (MIBI-TOF). Staining was performed as described with metal conjugate of this antibody: 165 Ho. Images were acquired using MIBIscope (Ionpath, Inc.).
Dr. Tal Keidar
Verified customer
Submitted Mar 10 2021