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  1. Link

    cd11c-antibody-ep1347y-c-terminal-ab52632.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)

  • Datasheet
  • SDS
Reviews (18)Q&A (10)References (64)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunoprecipitation - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
  • Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1347Y] to CD11c - C-terminal
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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View more associated products

Overview

  • Product name

    Anti-CD11c antibody [EP1347Y] - C-terminal
    See all CD11c primary antibodies
  • Description

    Rabbit monoclonal [EP1347Y] to CD11c - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate; THP-1 + TPA lysate. IHC-P: Human tonsil, lymph node and spleen tissues. IP: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1347Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • HRP Anti-CD11c antibody [EP1347Y] (ab195534)
    • Anti-CD11c antibody [EP1347Y] - BSA and Azide free (ab216655)
    • Alexa Fluor® 647 Anti-CD11c antibody [EP1347Y] (ab275338)
    • Alexa Fluor® 488 Anti-CD11c antibody [EP1347Y] (ab275738)
    • Alexa Fluor® 594 Anti-CD11c antibody [EP1347Y] (ab277235)
    • Alexa Fluor® 555 Anti-CD11c antibody [EP1347Y] (ab279329)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD11c protein (ab158776)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (12)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
IP
1/20.

For unpurified use at 1/80.

Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
IP
1/20.

For unpurified use at 1/80.

Target

  • Function

    Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P20702 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3687 Human
    • Omim: 151510 Human
    • SwissProt: P20702 Human
    • Unigene: 248472 Human
    • Alternative names

      • 95 alpha chain antibody
      • 95 antibody
      • CD 11c antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member C antibody
      • CD11c antibody
      • CD11c antigen antibody
      • Complement component 3 receptor 4 subunit antibody
      • CR4 antibody
      • Integrin alpha X antibody
      • Integrin alpha X chain antibody
      • Integrin alpha-X antibody
      • Integrin aX antibody
      • Integrin subunit alpha X antibody
      • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • integrin, alpha X (complement component 3 receptor 4 subunit antibody
      • ITAX_HUMAN antibody
      • ITGAX antibody
      • LEU M5 alpha subunit antibody
      • Leu M5 antibody
      • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
      • Leukocyte adhesion glycoprotein p150 antibody
      • Leukocyte adhesion receptor p150 95 antibody
      • Leukocyte adhesion receptor p150 antibody
      • Leukocyte surface antigen p150 95 alpha subunit antibody
      • Leukocyte surface antigen p150 alpha subunit antibody
      • Myeloid membrane antigen alpha subunit antibody
      • p150 95 integrin alpha chain antibody
      • p150 antibody
      • p150/95 antibody
      • SLEB6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD11c with Purified ab52632 at 1:500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (purified)

      Lane 1 : Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 128 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunoprecipitation - Anti-CD11c antibody [EP1347Y] (ab52632)

      ab52632 (purified) at 1:20 dilution (2µg) immunoprecipitating CD11c in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.

      Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate 10ug
      Lane 2 (+): ab52632 & THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52632 in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST."

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)Image courtesy of an anonymous abreview

      Immunohsitochemical analysis of paraffin-embedded human tonsil tissue labelling CD11c with ab52632 at 1/500 dilution. Tissue fixed with 10% NBF. An Alexa Fluor® 647 Goat anti-Rabbit IgG secondary antibody was used. Heat mediated antigen retrieval with Tris/EDTA, ph 9 carried out.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Unpurified ab52632 showing positive staining in Normal tonsil tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)This image is courtesy of an Abreview submitted by James Jupp

      Unpurified ab52632 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with ab52632 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Unpurified ab52632 showing negative staining in Normal brain tissue.

    • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified)

      Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 128 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?


      Exposure time: 4 minutes


      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52632 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      Western blot - Anti-CD11c antibody [EP1347Y] (ab52632)
      Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified) + THP-1 + TPA lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 128 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Unpurified ab52632 showing negative staining in Normal breast tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Unpurified ab52632 showing negative staining in Skeletal muscle tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] (ab52632)

      Unpurified ab52632 showing positive staining in Normal spleen tissue.

    • Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)
      Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (64)

    Publishing research using ab52632? Please let us know so that we can cite the reference in this datasheet.

    ab52632 has been referenced in 64 publications.

    • Pan C  et al. Phenotypic profiling and prognostic significance of immune infiltrates in esophageal squamous cell carcinoma. Oncoimmunology 10:1883890 (2021). PubMed: 33628625
    • Wessels JM  et al. Depot medroxyprogesterone acetate (DMPA) enhances susceptibility and increases the window of vulnerability to HIV-1 in humanized mice. Sci Rep 11:3894 (2021). PubMed: 33594113
    • Blight BJ  et al. Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis. J Asthma Allergy 14:585-593 (2021). PubMed: 34079296
    • Abdullahi A  et al. Adipose browning response to burn trauma is impaired with aging. JCI Insight 6:N/A (2021). PubMed: 34423787
    • Petrillo F  et al. Resolvin D1 attenuates the inflammatory process in mouse model of LPS-induced keratitis. J Cell Mol Med 24:12298-12307 (2020). PubMed: 33058526
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 28 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD11c antibody [EP1347Y] - C-terminal

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (vessel organoid)
    Permeabilization
    No
    Specification
    vessel organoid
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    MR. Jian Wu

    Verified customer

    Submitted Nov 22 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y] - C-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica ER2
    Permeabilization
    No
    Specification
    lymph node
    Fixative
    Formaldehyde
    Read More

    Mr. 勝啓 平田

    Verified customer

    Submitted Apr 01 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: acetic and EDTA tested
    Permeabilization
    No
    Specification
    mouse tumor
    Blocking step
    Serum as blocking agent for 8 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    No blocking step used for 10 minute(s) · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 09 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 09 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Sarcoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Human Sarcoma
    Blocking step
    Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 09 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    No
    Specification
    Lymph node
    Blocking step
    Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 03 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11c antibody [EP1347Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated
    Sample
    Rabbit Tissue sections (Tonsil)
    Specification
    Tonsil
    Permeabilization
    Yes - 0.2% Tween in PBS
    Fixative
    Formaldehyde
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    Submitted Nov 25 2014

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