Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21826] to CD11c
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse
Product nameAnti-CD11c antibody [EPR21826]
See all CD11c primary antibodies
DescriptionRabbit monoclonal [EPR21826] to CD11c
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD11c aa 150-350. The exact sequence is proprietary.
Database link: Q9QXH4
- IHC-P: Mouse spleen tissue. Flow Cyt: Mouse primary splenocytes.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab219799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11c with ab219799 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on dendritic cells of mouse spleen (PMID: 20194631, PMID: 15795237) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
Flow cytometric analysis of mouse primary splenocytes labeling CD11c with ab219799 at a 1/50 dilution.
Cells were stained with BV421 conjugated CD11b and rabbit IgG (Left panel) or CD11c (Right panel).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219799 has not yet been referenced specifically in any publications.