Recombinant Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD11c antibody [EPR21826] - BSA and Azide free
See all CD11c primary antibodies -
Description
Rabbit monoclonal [EPR21826] to CD11c - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD11c aa 150-350. The exact sequence is proprietary.
Database link: Q9QXH4 -
Positive control
- IHC-P: Mouse spleen tissue.
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General notes
Ab240558 is the carrier-free version of ab219799. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240558 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21826 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Immunohistochemistry reagents
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16411 Mouse
- SwissProt: Q9QXH4 Mouse
- Unigene: 22378 Mouse
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Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
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![Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-320488-anti-cd11c-antibody-epr21826-flow-cytometry.jpg)
Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)Flow cytometric analysis of mouse primary splenocytes labeling CD11c with ab219799 at a 1/50 dilution.
Cells were stained with BV421 conjugated CD11b and rabbit IgG (Left panel) or CD11c (Right panel).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219799).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-319063-anti-cd11c-antibody-epr21826-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11c with ab219799 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on dendritic cells of mouse spleen (PMID: 20194631, PMID: 15795237) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab219799).
Datasheets and documents
References
ab240558 has not yet been referenced specifically in any publications.
