Recombinant Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
![Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-320488-anti-cd11c-antibody-epr21826-flow-cytometry.jpg "Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21826] to CD11c - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Mouse
Overview
-
Product name
Anti-CD11c antibody [EPR21826] - BSA and Azide free
See all CD11c primary antibodies -
Description
Rabbit monoclonal [EPR21826] to CD11c - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment within Mouse CD11c aa 150-350. The exact sequence is proprietary.
Database link: Q9QXH4 -
Positive control
- IHC-P: Mouse spleen tissue.
-
General notes
Ab240558 is the carrier-free version of ab219799. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab240558 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21826 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Immunohistochemistry kits
-
Immunohistochemistry reagents
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab240558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
-
Function
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 16411 Mouse
- SwissProt: Q9QXH4 Mouse
- Unigene: 22378 Mouse
-
Alternative names
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
Images
-
Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
Flow cytometric analysis of mouse primary splenocytes labeling CD11c with ab219799 at a 1/50 dilution.
Cells were stained with BV421 conjugated CD11b and rabbit IgG (Left panel) or CD11c (Right panel).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219799).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-319063-anti-cd11c-antibody-epr21826-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11c with ab219799 at 1/100 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on dendritic cells of mouse spleen (PMID: 20194631, PMID: 15795237) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab219799).
-
![Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)](https://www.abcam.com/ps/products/240/ab240558/Images/ab240558-3-benefits-of-recombinant-antibodies.png)
Anti-CD11c antibody [EPR21826] - BSA and Azide free (ab240558)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab240558 has not yet been referenced specifically in any publications.
