Key features and details
- Mouse monoclonal [ITGAX/1243] to CD11c
- Suitable for: Protein Array, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-CD11c antibody [ITGAX/1243]
See all CD11c primary antibodies
DescriptionMouse monoclonal [ITGAX/1243] to CD11c
Tested applicationsSuitable for: Protein Array, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CD11c aa 637-827.
FECR EQVVSEQTLV QSNICLYIDK RSKNLLGSRD LQSSVTLDLA LDPGRLSPRA TFQETKNRSL SRVRVLGLKA HCENFNLLLP SCVEDSVTPI TLRLNFTLVG KPLLAFRNLR PMLAADAQRY FTASLPFEKN CGADHICQDN LGISFSFPGL KSLLVGSNLE LNAEVMVWND GEDSYGTTIT FSHPAGL
Database link: P20702
- IHC-P: Human prostate carcinoma, lung carcinoma and follicular lymphoma tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab215858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5-1µl for 106 cells.|
|IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
ab215858 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human follicular lympmoma tissue labeling CD11c with ab215858 at 1 µg/mL.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling CD11c with ab215858 at 1 µg/mL.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD11c with ab215858 at 1 µg/mL.
ab215858 has not yet been referenced specifically in any publications.