Product nameAnti-CD11c antibody [N418]
See all CD11c primary antibodies
DescriptionArmenian hamster monoclonal [N418] to CD11c
Host speciesArmenian hamster
Specificityab33483 recognizes the murine homologue of human CD11c (150/90kD) integrin family. This product is recommended for IHC-Fr only although some customers have used it successfully in IHC-P.
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IPmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse CD11c.
- IHC: mouse liver tissue, mouse spleen tissue Flow Cyt: Mouse splenocytes.
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein G purified
Primary antibody notesClone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
- Anti-CD11c antibody [N418] (PerCP/Cy5.5®) (ab111469)
- Anti-CD11c antibody [N418] - Low endotoxin, Azide free (ab185783)
- Anti-CD11c antibody [N418] (Allophycocyanin) (ab210306)
- Anti-CD11c antibody [N418] (Allophycocyanin/Cy7 ®) (ab210307)
- Anti-CD11c antibody [N418] (FITC) (ab210308)
- Anti-CD11c antibody [N418] (Phycoerythrin) (ab210309)
- Anti-CD11c antibody [N418] (PE/Cy7 ®) (ab210310)
- Anti-CD11c antibody [N418] (Alexa Fluor® 647) (ab233046)
- Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
- Anti-CD11c antibody [N418] (violetFluor™ 450) (ab242269)
- Anti-CD11c antibody [N418] (Alexa Fluor® 488) (ab33503)
- Anti-CD11c antibody [N418] (Biotin) (ab95765)
Our Abpromise guarantee covers the use of ab33483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20808846|
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.
Abcam recommends using acetone fixation.
|IP||Use at an assay dependent concentration.|
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.
Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.
ab33483 staining CD11c in murine bronchoalveolar lavage (BAL) cells by Immunocytochemistry/ Immunofluorescence.
BAL cells were collected from PBS treated mice at day 13, 17 or 18 (17/18) and 21 post-reconstitution. Cells were stained with ab33483 specific for CD11c (green) and Pc (red). Representative images of brightfield (BF), CD11c, Pc, and merged CD11c/Pc are shown for PBS-treated mice following immune reconstitution Day 21.
This product has been referenced in:
- Zheng J et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). Read more (PubMed: 29848296) »
- Yin Y et al. The homing of human umbilical cord-derived mesenchymal stem cells and the subsequent modulation of macrophage polarization in type 2 diabetic mice. Int Immunopharmacol 60:235-245 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29778021) »