Key features and details
- Armenian hamster monoclonal [N418] to CD11c (Alexa Fluor® 488)
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG
Product nameAnti-CD11c antibody [N418] (Alexa Fluor® 488)
See all CD11c primary antibodies
DescriptionArmenian hamster monoclonal [N418] to CD11c (Alexa Fluor® 488)
Host speciesArmenian hamster
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue, cells or virus corresponding to Mouse CD11c.
- Flow Cyt: C57BL/6 mouse splenocytes.
Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Anti-CD11c antibody [N418] (PerCP/Cy5.5®) (ab111469)
- Anti-CD11c antibody [N418] - Low endotoxin, Azide free (ab185783)
- Anti-CD11c antibody [N418] (APC) (ab210306)
- Anti-CD11c antibody [N418] (APC/Cy7®) (ab210307)
- Anti-CD11c antibody [N418] (FITC) (ab210308)
- Anti-CD11c antibody [N418] (PE) (ab210309)
- Anti-CD11c antibody [N418] (PE/Cy7®) (ab210310)
- Anti-CD11c antibody [N418] (Alexa Fluor® 647) (ab233046)
- Anti-CD11c antibody [N418] - BSA and Azide free (ab233173)
- Anti-CD11c antibody [N418] (violetFluor™ 450) (ab242269)
- Anti-CD11c antibody [N418] (ab33483)
- Anti-CD11c antibody [N418] (Biotin) (ab95765)
Our Abpromise guarantee covers the use of ab33503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionIntegrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Tissue specificityPredominantly expressed in monocytes and granulocytes.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
C57BL/6 mouse splenocytes stained with ab33503 (right) or Armenian Hamster IgG Alexa Fluor® 488 (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab33503) or Armenian Hamster IgG 488 Isotype (1x106 in 100 µl at 1:500 (1 µg/ml)) for 30 min on ice. The cells were simultaneously stained with the following conjugated antibodies: MHC Class II PerCP, CD19 APC and CD3 PE.
Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Gating strategy: T and B cells were excluded from viable lymphocytes and expression of MHC Class II and CD11c was assessed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab33503 has not yet been referenced specifically in any publications.