Overview

  • Product name

    Anti-CD11c antibody [N418] - BSA and Azide free
    See all CD11c primary antibodies
  • Description

    Armenian hamster monoclonal [N418] to CD11c - BSA and Azide free
  • Host species

    Armenian hamster
  • Specificity

    ab233173 recognizes the murine homologue of human CD11c (150/90kD) integrin family. This product is recommended for IHC-Fr only although some customers have used it successfully in IHC-P.

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD11c.

  • Positive control

    • Flow Cyt: Murine alveolar cells. Mouse splenic cells
  • General notes

    ab233173 is the PBS only version of ab33483.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Properties

Applications

Our Abpromise guarantee covers the use of ab233173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
    • Tissue specificity

      Predominantly expressed in monocytes and granulocytes.
    • Sequence similarities

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
      Contains 1 VWFA domain.
    • Domain

      The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 95 alpha chain antibody
      • 95 antibody
      • CD 11c antibody
      • CD11 antigen like family member C antibody
      • CD11 antigen-like family member C antibody
      • CD11c antibody
      • CD11c antigen antibody
      • Complement component 3 receptor 4 subunit antibody
      • CR4 antibody
      • Integrin alpha X antibody
      • Integrin alpha X chain antibody
      • Integrin alpha-X antibody
      • Integrin aX antibody
      • Integrin subunit alpha X antibody
      • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
      • integrin, alpha X (complement component 3 receptor 4 subunit antibody
      • ITAX_HUMAN antibody
      • ITGAX antibody
      • LEU M5 alpha subunit antibody
      • Leu M5 antibody
      • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
      • Leukocyte adhesion glycoprotein p150 antibody
      • Leukocyte adhesion receptor p150 95 antibody
      • Leukocyte adhesion receptor p150 antibody
      • Leukocyte surface antigen p150 95 alpha subunit antibody
      • Leukocyte surface antigen p150 alpha subunit antibody
      • Myeloid membrane antigen alpha subunit antibody
      • p150 95 integrin alpha chain antibody
      • p150 antibody
      • p150/95 antibody
      • SLEB6 antibody
      see all

    Images

    • Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labeling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000.

      Gating strategy was against CD45+, C11b+, GR1-cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

    • Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488)  preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

      Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

    References

    ab233173 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab233173.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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