The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 105-8 cells.
In a final volume of 100 μL.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
Predominantly expressed in monocytes and granulocytes.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. Contains 1 VWFA domain.
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Flow cytometric analysis of mouse C57BI/6 splenocytes with an anti-Human/mouse CD45R antibody and 0.5 µg of armenian hamster IgG Isotype Control (left) or 0.5 µg of ab185783 (right) followed by anti-armenian hamster IgG FITC. Cells in the lymphocyte gate were used for analysis.