Anti-CD11+CD18 antibody [24] (ab13219)

Reviews (5)Q&A (3)References (16)

Key features and details

  • Mouse monoclonal [24] to CD11+CD18
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD11+CD18 antibody [24]
    See all CD11+CD18 primary antibodies

  • Description

    Mouse monoclonal [24] to CD11+CD18

  • Host species

    Mouse

  • Specificity

    This antibody specifically recognises the human CD11+CD18 activation epitope.

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Fibronectin-purified human monocytes.

  • Epitope

    Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.

Properties

Target

  • Function

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

  • Tissue specificity

    Leukocytes.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
    • CD11 antigen-like family member A antibody
    • CD11a antibody
    • CD18 antibody
    • CR3 antibody
    • Integrin alpha-L antibody
    • Integrin beta 2 antibody
    • Integrin gene promoter antibody
    • ITAL_HUMAN antibody
    • Itgal antibody
    • ITGAM antibody
    • ITGB2 antibody
    • LAD antibody
    • Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody
    • Leukocyte function-associated molecule 1 alpha chain antibody
    • LFA 1 alpha antibody
    • LFA 1 antibody
    • LFA-1A antibody
    • LFA1 antibody
    • LFA1A antibody
    • Lymphocyte function associated antigen 1 antibody
    • MAC1 antibody
    • MFI7 antibody
    • MO1A antibody
    see all

References (16)

Publishing research using ab13219? Please let us know so that we can cite the reference in this datasheet.

ab13219 has been referenced in 16 publications.

  • Chen Q  et al. Leukocyte-mimicking Pluronic-lipid nanovesicle hybrids inhibit the growth and metastasis of breast cancer. Nanoscale 11:5377-5394 (2019). PubMed: 30849160
  • Drewry LL  et al. The secreted kinase ROP17 promotes Toxoplasma gondii dissemination by hijacking monocyte tissue migration. Nat Microbiol 4:1951-1963 (2019). PubMed: 31332383
  • Yuan X  et al. Recipient treatment with acetylcholinesterase inhibitor donepezil attenuates primary graft failure in rats through inhibiting post-transplantational donor heart ischaemia/reperfusion injury. Eur J Cardiothorac Surg 53:400-408 (2018). PubMed: 28950359
  • Laufer JM  et al. CCR7 Is Recruited to the Immunological Synapse, Acts as Co-stimulatory Molecule and Drives LFA-1 Clustering for Efficient T Cell Adhesion Through ZAP70. Front Immunol 9:3115 (2018). PubMed: 30692994
  • Manwani D  et al. Single-dose intravenous gammaglobulin can stabilize neutrophil Mac-1 activation in sickle cell pain crisis. Am J Hematol 90:381-5 (2015). Flow Cyt ; Human . PubMed: 25616042
View all Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Question



I need for a flow cytometry experiment using Anti-CD11+CD18 antibody [24] (ab13219) against LFA1 on THP1 cells. Please suggest Isotype controls.




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Answer

The Anti-CD11+CD18 antibody [24] (ab13219) is a mouse IgG1 monoclonal antibody.
We have the following Isotype control that you can use for your flow cytometry experiment:
Mouse IgG1 [CT6] - Isotype control (ab81216), tested applications: Flow Cyr,IHC-P and WB,
https://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html https://www.abcam.com/Mouse-IgG1-CT6-Isotype-control-ab81216.html.
This antibody has been referenced in 2 publications that you can visualize by clicking the following link:
https://www.abcam.com/mouse-igg1-ct6-isotype-control-ab81216-references.html
You can also find Isotype controls from our homepage by selecting the Control tab (top right) and selecting Isotype controls for the drop-down menu. You can use search filters on the left hand site to help find a suitable control for your experiments.

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Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More

Abcam user community

Verified customer

Submitted Nov 29 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Dog Cell (blood leucocyte)
Specification
blood leucocyte
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Read More

Abcam user community

Verified customer

Submitted Nov 12 2012

Question

Many thanks for your quick reply.

Can I just confirm that the antibody specifically recognizes active CD11a/CD18 (LFA-1) or does it all recognize active CD11b/c/d with CD18?

Thanks,

Read More
Answer

Thank you for your patience. My colleague is out of office this week.

I regret to inform you that we do not have any direct data that answers your question. However,we know the scientist described the antibody as being able to be used to detect the activation state of each integrin receptor which suggests that it does recognize CD11a, b, c and d.

Apologies! If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Product code: 13219
Inquiry: I am looking for an antibody that will be able to distinguish between inactive and active LFA-1 (CD11a/CD18) expression. The data sheets suggest that the antibody is to an epitope on CD18 that is only exposed once it has dimerised with the alpha chain and the integrin has been activated? Is this specific for active LFA-1 or beta 2 integrins in general?

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Answer

Thank you for contacting us.

The antibody was found to recognize a unique activation epitope of LFA-1, which is essentially absent on resting cells e.g. lymphocytes so this product may be used as a reporter of the activation state of CD11/CD18 receptors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Poor
Abreviews
abreview image
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More

Abcam user community

Verified customer

Submitted May 04 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: after Ficoll
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
monocytes
Read More

Abcam user community

Verified customer

Submitted Nov 18 2011

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Permeabilization
No
Read More

Abcam user community

Verified customer

Submitted Oct 28 2011

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