Anti-CD11+CD18 antibody [24] (ab13219)

Reviews (5)Q&A (3)References (12)

Overview

  • Product name
    Anti-CD11+CD18 antibody [24]
    See all CD11+CD18 primary antibodies
  • Description
    Mouse monoclonal [24] to CD11+CD18
  • Host species
    Mouse
  • Specificity
    This antibody specifically recognises the human CD11+CD18 activation epitope.
  • Tested applications
    Suitable for: RIA, ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Fibronectin-purified human monocytes.

  • Epitope
    Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.

Properties

Applications

Our Abpromise guarantee covers the use of ab13219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 22473604

Target

Images

  • Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)
    Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)Image courtesy of an anonymous Abreview.
    ab13219 staining CD11 + CD18 in human PBMC by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. An FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)
    Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)This image is courtesy of an anonymous Abreview
    ab13219 staining CD11 + CD18 in Mouse splenocytes by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. A FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.

References

This product has been referenced in:
  • Manwani D  et al. Single-dose intravenous gammaglobulin can stabilize neutrophil Mac-1 activation in sickle cell pain crisis. Am J Hematol 90:381-5 (2015). Flow Cyt ; Human . Read more (PubMed: 25616042) »
  • Tittarelli A  et al. Gap junction intercellular communications regulate NK cell activation and modulate NK cytotoxic capacity. J Immunol 192:1313-9 (2014). Human . Read more (PubMed: 24376266) »
See all 12 Publications for this product

Publishing research using ab13219? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Question



I need for a flow cytometry experiment using Anti-CD11+CD18 antibody [24] (ab13219) against LFA1 on THP1 cells. Please suggest Isotype controls.




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Answer

The Anti-CD11+CD18 antibody [24] (ab13219) is a mouse IgG1 monoclonal antibody.
We have the following Isotype control that you can use for your flow cytometry experiment:
Mouse IgG1 [CT6] - Isotype control (ab81216), tested applications: Flow Cyr,IHC-P and WB,
https://www.abcam.com/index.html?datasheet=81216 https://www.abcam.com/index.html?datasheet=81216.
This antibody has been referenced in 2 publications that you can visualize by clicking the following link:
https://www.abcam.com/mouse-igg1-ct6-isotype-control-ab81216-references.html
You can also find Isotype controls from our homepage by selecting the Control tab (top right) and selecting Isotype controls for the drop-down menu. You can use search filters on the left hand site to help find a suitable control for your experiments.

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Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More

Abcam user community

Verified customer

Submitted Nov 29 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Flow Cytometry
Sample
Dog Cell (blood leucocyte)
Specification
blood leucocyte
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
monocytes
Read More

Abcam user community

Verified customer

Submitted Nov 12 2012

Question

Many thanks for your quick reply.

Can I just confirm that the antibody specifically recognizes active CD11a/CD18 (LFA-1) or does it all recognize active CD11b/c/d with CD18?

Thanks,

Read More
Answer

Thank you for your patience. My colleague is out of office this week.

I regret to inform you that we do not have any direct data that answers your question. However,we know the scientist described the antibody as being able to be used to detect the activation state of each integrin receptor which suggests that it does recognize CD11a, b, c and d.

Apologies! If you need any further assistance in the future, please do not hesitate to contact me.

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Question

Product code: 13219
Inquiry: I am looking for an antibody that will be able to distinguish between inactive and active LFA-1 (CD11a/CD18) expression. The data sheets suggest that the antibody is to an epitope on CD18 that is only exposed once it has dimerised with the alpha chain and the integrin has been activated? Is this specific for active LFA-1 or beta 2 integrins in general?

Read More
Answer

Thank you for contacting us.

The antibody was found to recognize a unique activation epitope of LFA-1, which is essentially absent on resting cells e.g. lymphocytes so this product may be used as a reporter of the activation state of CD11/CD18 receptors.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
abreview image
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More

Abcam user community

Verified customer

Submitted May 04 2012

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: after Ficoll
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
monocytes
Read More

Abcam user community

Verified customer

Submitted Nov 18 2011

Flow Cytometry abreview for Anti-CD11+CD18 antibody [24]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Permeabilization
No
Read More

Abcam user community

Verified customer

Submitted Oct 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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