- Datasheet
- Specific References (12)
- Protocols
Overview
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Product nameAnti-CD11+CD18 antibody [24]
See all CD11+CD18 primary antibodies -
DescriptionMouse monoclonal [24] to CD11+CD18
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Host speciesMouse
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SpecificityThis antibody specifically recognises the human CD11+CD18 activation epitope.
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Tested applicationsSuitable for: RIA, ICC/IF, Flow Cyt, IP, WBmore details
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Species reactivityReacts with: Mouse, Rat, Dog, Human
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Immunogen
Fibronectin-purified human monocytes.
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EpitopeRecognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityProtein A/G purified
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ClonalityMonoclonal
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Clone number24
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MyelomaSp2/0-Ag14
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IsotypeIgG1
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab13219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| RIA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. PubMed: 22473604 |
Target
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FunctionIntegrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.
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Tissue specificityLeukocytes.
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Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3683 Human
- Entrez Gene: 3684 Human
- Entrez Gene: 3687 Human
- Entrez Gene: 3689 Human
- Entrez Gene: 16408 Mouse
- Entrez Gene: 16409 Mouse
- Entrez Gene: 16414 Mouse
- Entrez Gene: 25021 Rat
see all -
Alternative names
- Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody
- CD11 antigen-like family member A antibody
- CD11a antibody
see all
Images
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![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)](https://www.abcam.com/ps/products/13/ab13219/Images/CD11CD18-Primary-antibodies-ab13219-17.jpg)
Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)Image courtesy of an anonymous Abreview.ab13219 staining CD11 + CD18 in human PBMC by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. An FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody. -
![Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)](https://www.abcam.com/ps/products/13/ab13219/Images/CD11CD18-Primary-antibodies-ab13219-9.jpg)
Flow Cytometry - Anti-CD11+CD18 antibody [24] (ab13219)This image is courtesy of an anonymous Abreviewab13219 staining CD11 + CD18 in Mouse splenocytes by Flow Cytometry. Cells were prepared in PBS + 1% BSA. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA) for 1 hour at 4°C. A FITC-conjugated Goat anti-mouse polyclonal (1/200) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Manwani D et al. Single-dose intravenous gammaglobulin can stabilize neutrophil Mac-1 activation in sickle cell pain crisis. Am J Hematol 90:381-5 (2015). Flow Cyt ; Human . Read more (PubMed: 25616042) »
- Tittarelli A et al. Gap junction intercellular communications regulate NK cell activation and modulate NK cytotoxic capacity. J Immunol 192:1313-9 (2014). Human . Read more (PubMed: 24376266) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"