Anti-CD127 antibody [A7R34] (PE/Cy7 ®) (ab210242)

Overview

  • Product name
    Anti-CD127 antibody [A7R34] (PE/Cy7 ®)
    See all CD127 primary antibodies
  • Description
    Rat monoclonal [A7R34] to CD127 (PE/Cy7 ®)
  • Host species
    Rat
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • C57Bl/6 splenocytes.
  • General notes

    The A7R34 antibody may be used as a phenotypic marker for IL7R alpha (CD127) on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8-) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells.

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

Properties

Applications

Our Abpromise guarantee covers the use of ab210242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use 0.5 µg.

Target

  • Function
    Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
  • Involvement in disease
    Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
  • Sequence similarities
    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications
    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 127 antibody
    • CD127 antibody
    • CD127 antigen antibody
    • CDw127 antibody
    • IL 7R alpha antibody
    • IL 7R antibody
    • IL-7 receptor subunit alpha antibody
    • IL-7R subunit alpha antibody
    • IL-7R-alpha antibody
    • IL-7RA antibody
    • IL7R antibody
    • IL7RA antibody
    • IL7RA_HUMAN antibody
    • IL7Ralpha antibody
    • ILRA antibody
    • Interleukin 7 receptor alpha chain antibody
    • Interleukin 7 receptor antibody
    • Interleukin 7 receptor isoform H5 6 antibody
    • Interleukin-7 receptor subunit alpha antibody
    see all

Images

  • Flow cytometric analysis of C57Bl/6 splenocytes stained with FITC Anti-Mouse CD3, labeling CD127 with 0.5 μg ab210242 (right panel), compared with 0.5 μg PE-Cy7 Rat IgG2a isotype control (left panel).

References

ab210242 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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