Key features and details
- Rabbit polyclonal to CD127
- Suitable for: ELISA, Dot blot
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-CD127 antibody
See all CD127 primary antibodies
DescriptionRabbit polyclonal to CD127
Tested applicationsSuitable for: ELISA, Dot blotmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Synthetic peptide corresponding to Mouse CD127 (C terminal).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab95024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Involvement in diseaseDefects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsN-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD 127 antibody
- CD127 antibody
- CD127 antigen antibody
Dot Blot - Anti-CD127 antibody (ab95024).
Antigen: Immunizing peptide, phosphorylated version of immunizing peptide and THRB1 and THRB1 pY171 as controls.
Load: 3.0, 0.3, 0.1, 0.05 µg as indicated.
Primary antibody: ab95024 at 1/400 for 45 min at 4°C.
Secondary antibody: Dylight™488 rabbit at 1/10,000 for 45 min at RT.
Block: 5% BLOTTO overnight at 4°C.
ELISA results of ab95024 tested against BSA-conjugated peptide of immunizing peptide.
Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody-Peroxidase and TMB ELISA peroxidase substrate.
ab95024 has been referenced in 1 publication.
- Jiang H et al. Elevated IL-7Ra is linked to recurrence and poorer survival of gastric adenocarcinoma. Int J Clin Exp Pathol 11:1645-1652 (2018). PubMed: 31938264