Overview

  • Product name

    Anti-CD127 antibody [EPR2955(2)]
    See all CD127 primary antibodies
  • Description

    Rabbit monoclonal [EPR2955(2)] to CD127
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD127 aa 350-450. The exact sequence is proprietary.
    Database link: P16871

  • Positive control

    • K562, HeLa, Daudi and Jurkat whole cell lysate (ab7899), K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 52 kDa).
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
  • Involvement in disease

    Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 127 antibody
    • CD127 antibody
    • CD127 antigen antibody
    • CDw127 antibody
    • IL 7R alpha antibody
    • IL 7R antibody
    • IL-7 receptor subunit alpha antibody
    • IL-7R subunit alpha antibody
    • IL-7R-alpha antibody
    • IL-7RA antibody
    • IL7R antibody
    • IL7RA antibody
    • IL7RA_HUMAN antibody
    • IL7Ralpha antibody
    • ILRA antibody
    • Interleukin 7 receptor alpha chain antibody
    • Interleukin 7 receptor antibody
    • Interleukin 7 receptor isoform H5 6 antibody
    • Interleukin-7 receptor subunit alpha antibody
    see all

Images

  • Flow cytometric analysis of K562 cells (2% paraformaldehyde-fixed) labelingCD127 with ab180521 at 1/200 dilution (red) compared with a Rabbit monoclonal IgG negative control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/75 dilution.

  • All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 52 kDa

  • All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysates
    Lane 2 : Mouse heart tissue lysates
    Lane 3 : Mouse kidney tissue lysates
    Lane 4 : Rat brain tissue lysates
    Lane 5 : Rat heart tissue lysates
    Lane 6 : Rat kidney tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 52 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST 

  • All lanes : Anti-CD127 antibody [EPR2955(2)] (ab180521) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 52 kDa

References

This product has been referenced in:

  • Lei X  et al. Down-regulation of interleukin 7 receptor (IL-7R) contributes to central nervous system demyelination. Oncotarget 8:28395-28407 (2017). Read more (PubMed: 28415697) »
See 1 Publication for this product

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