Overview

  • Product name

    Anti-CD127 antibody [MM0411-10V5]
    See all CD127 primary antibodies
  • Description

    Mouse monoclonal [MM0411-10V5] to CD127
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CD127 (extracellular).

Properties

Applications

Our Abpromise guarantee covers the use of ab95023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
  • Involvement in disease

    Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 1 fibronectin type-III domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 127 antibody
    • CD127 antibody
    • CD127 antigen antibody
    • CDw127 antibody
    • IL 7R alpha antibody
    • IL 7R antibody
    • IL-7 receptor subunit alpha antibody
    • IL-7R subunit alpha antibody
    • IL-7R-alpha antibody
    • IL-7RA antibody
    • IL7R antibody
    • IL7RA antibody
    • IL7RA_HUMAN antibody
    • IL7Ralpha antibody
    • ILRA antibody
    • Interleukin 7 receptor alpha chain antibody
    • Interleukin 7 receptor antibody
    • Interleukin 7 receptor isoform H5 6 antibody
    • Interleukin-7 receptor subunit alpha antibody
    see all

References

ab95023 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us. I apologize for the delay. The catalogue number of blocking peptide is ab116167. The price is £110 for 100 ug size. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. The positive controls for ab95023 are cell lysates of A-431, U2OS, U251 MG, Hela cell etc. Please click the following to see the positive control tissue lysates; http://www.proteinatlas.org/ENSG00000168685 We unfortunately cannot offer blocking peptide for this antibody; the peptide is not commercially available. We can certainly offer a blocking peptide for ab103404; let me know if you are interested in buying this we will then publish this product as special request. TLR4 antibody:- We can recommend ab83444; https://www.abcam.com/tlr4-antibody-ab83444.html The blocking peptide for this is ab93304; https://www.abcam.com/TLR4-peptide-ab93304.html The positive control lysates would be Human, Mouse and Rat small intestine; NIH3T3; EL4 etc. CD45 antibody and peptide would be:- ab10559; https://www.abcam.com/CD45-antibody-ab10559.html and ab17550; https://www.abcam.com/CD45-peptide-ab17550.html The positive control will be Jurkat, KM-H2, or human tonsil tissue lysates. Please also check human tissue Atlas for more information about positive control. Polyclonal antibodies can be used for protein quantifications also however because monoclonal antibodies binds more specifically to particular proteins so monoclonal antibodies are recommended more than polyclonal antibodies. The choice antibodies are depends on the type of application used e.g. in sandwich ELISA monoclonal is best. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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