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Immunology Innate Immunity Cytokines Interleukins
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Anti-CD127 (phospho Y449) antibody (ab118527)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127 (phospho Y449) antibody (ab118527)

    Key features and details

    • Rabbit polyclonal to CD127 (phospho Y449)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CD127 (phospho Y449) antibody
      See all CD127 primary antibodies
    • Description

      Rabbit polyclonal to CD127 (phospho Y449)
    • Host species

      Rabbit
    • Specificity

      ab118527 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Mouse CD127.
      Database link: NP_002176.2

    • Positive control

      • IHC-P: Human Colon, MALT lymphoma tissue.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.20
      Preservative: 0.01% Sodium azide
      Constituents: 0.424% Potassium phosphate, 0.88% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Cytokines
      • Interleukins
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human CD127 protein (Fc Chimera Active) (ab174078)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab118527 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
    • Involvement in disease

      Defects in IL7R are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
      Genetic variations in IL7R are a cause of susceptibility to multiple sclerosis type 3 (MS3) [MIM:612595]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=A polymorphism at position 244 strongly influences susceptibility to multiple sclerosis. Overtransmission of the major 'C' allele coding for Thr-244 is detected in offspring affected with multiple sclerosis. In vitro analysis of transcripts from minigenes containing either 'C' allele (Thr-244) or 'T' allele (Ile-244) shows that the 'C' allele results in an approximately two-fold increase in the skipping of exon 6, leading to increased production of a soluble form of IL7R. Thus, the multiple sclerosis associated 'C' risk allele of IL7R would probably decrease membrane-bound expression of IL7R. As this risk allele is common in the general population, some additional triggers are probably required for the development and progression of MS.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 4 subfamily.
      Contains 1 fibronectin type-III domain.
    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      modifications

      N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P16871 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3575 Human
      • Omim: 146661 Human
      • SwissProt: P16871 Human
      • Unigene: 591742 Human
      • Unigene: 635723 Human
      • Alternative names

        • CD 127 antibody
        • CD127 antibody
        • CD127 antigen antibody
        • CDw127 antibody
        • IL 7R alpha antibody
        • IL 7R antibody
        • IL-7 receptor subunit alpha antibody
        • IL-7R subunit alpha antibody
        • IL-7R-alpha antibody
        • IL-7RA antibody
        • IL7R antibody
        • IL7RA antibody
        • IL7RA_HUMAN antibody
        • IL7Ralpha antibody
        • ILRA antibody
        • Interleukin 7 receptor alpha chain antibody
        • Interleukin 7 receptor antibody
        • Interleukin 7 receptor isoform H5 6 antibody
        • Interleukin-7 receptor subunit alpha antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127 (phospho Y449) antibody (ab118527)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127 (phospho Y449) antibody (ab118527)
        Human Colon, MALT lymphoma tissue (formalin-fixed, paraffin-embedded) stained with ab118527 at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab118527? Please let us know so that we can cite the reference in this datasheet.

      ab118527 has not yet been referenced specifically in any publications.

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