Validated using a knockout cell line

Recombinant Anti-CD13 antibody [EPR4058] (ab108310)

Knockout Tested Rabbit recombinant monoclonal CD13 antibody [EPR4058]. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 5 publication(s). Independently reviewed in 5 review(s).


  • Product name
    Anti-CD13 antibody [EPR4058]
    See all CD13 primary antibodies
  • Description
    Rabbit monoclonal [EPR4058] to CD13
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD13 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • THP-1 cell lysate, human fetal liver lysate, human kidney tissue
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • Tissue specificity
      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • Sequence similarities
      Belongs to the peptidase M1 family.
    • Domain
      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • Post-translational
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • Cellular localization
      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all


    • Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD13 (KO) whole cell lysate (20 µg)
      Lane 4: Empty
      Lane 5: HeLa whole cell lysate (20 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE.  Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Confocal image showing membranous staining in THP-1 cells

      ab108310 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as a counter stain and DAPI was used as a nuclear counter stain. 

    • All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate at 10 µg
      Lane 2 : human fetal liver lysate

      Performed under reducing conditions.

      Predicted band size: 110 kDa

    • ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
    • ab108310 showing positive staining in Normal liver tissue.

    • ab108310 showing positive staining in Hepatocellular carcinoma tissue.

    • ab108310 showing positive staining in Astrocytoma tissue.

    • ab108310 showing positive staining in Normal breast tissue.

    • ab108310 showing positive staining in Prostatic carcinoma tissue.

    • ab108310 showing positive staining in Normal tonsil tissue.

    • ab108310 showing positive staining in Normal stomach tissue.

    • ab108310 showing positive staining in Normal colon tissue.

    • ab108310 showing positive staining in Lung adenocarcinoma tissue.


    This product has been referenced in:
    • Harada K  et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
    • Nefla M  et al. The pro-inflammatory cytokine 14-3-3e is a ligand of CD13 in cartilage. J Cell Sci 128:3250-62 (2015). IHC . Read more (PubMed: 26208633) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-7 of 7 Abreviews or Q&A

    Pig Cultured Cells (Vero cells)
    Vero cells

    Mahesh Bhandari

    Verified customer

    Submitted Nov 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sheep Tissue sections (trochlea bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    trochlea bone
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jun 06 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Rhesus monkey Tissue sections (Jejunum)
    Antigen retrieval step

    Dr. Li Li

    Verified customer

    Submitted May 31 2017

    Immunocytochemistry/ Immunofluorescence
    Pig Cell (brain pericytes)
    brain pericytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C

    Mrs. Verena Ledwig

    Verified customer

    Submitted Mar 09 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Normal kidney
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3%

    Abcam user community

    Verified customer

    Submitted Feb 24 2016


    Thank you or your inquiry. I am happy to confirm that ab108310 (Anti-CD13 antibody [EPR4058]) is tested and guaranteed for ICC (Immunocytochemistry, which includes cell smears) and IHC-P with Mouse, Rat and Human samples. This antibody has not yet been tested for IHC-Fr and is therefore not covered by our guarantee for IHC-Fr. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program. To find out more about this please follow this link: Otherwise, we would like to encourage you to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints that can be used for discounts on future purchases. To find out more about our Abreview system, please see the following link: I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.

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    Thank you for contacting us. Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that storing our antibodies at various temperatures of up to 45°C for 1 week does not impact their activity. I would be happy to share a summary of our findings with you; please let me know if you would like to see this. As a precautionary measure only, we ship our antibodies in packaging with ice packs to provide extra temperature stability in transit. The ice pack may be thawed or even at room temperature when you receive it; please be assured that this is normal and that your product is safe to use. Once you have received the vial, please follow the long-term storage instructions on the datasheet. Our Abpromise to you is that, should our product not work as stated on the datasheet, we will resolve the issue to your satisfaction, either with helpful advice to optimize your experiment or a replacement or refund of the product. Please contact our knowledgeable Scientific Support staff should you experience any issues while using our products. Contact information can be found at: I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. PS: Attached is the tested data of few of our antibodies.

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