Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CD13 antibody [EPR4058] (ab108310)

Knockout Tested Rabbit recombinant monoclonal CD13 antibody [EPR4058]. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Human. Cited in 5 publication(s). Independently reviewed in 5 review(s).

Reviews (5)Q&A (2)References (5)

Overview

  • Product name
    Anti-CD13 antibody [EPR4058]
    See all CD13 primary antibodies
  • Description
    Rabbit monoclonal [EPR4058] to CD13
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CD13 aa 400-500. The exact sequence is proprietary.

  • Positive control
    • THP-1 cell lysate, human fetal liver lysate, human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • Tissue specificity
      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • Sequence similarities
      Belongs to the peptidase M1 family.
    • Domain
      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • Post-translational
      modifications
      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • Cellular localization
      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Images

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD13 (KO) whole cell lysate (20 µg)
      Lane 4: Empty
      Lane 5: HeLa whole cell lysate (20 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE.  Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] (ab108310)

      Confocal image showing membranous staining in THP-1 cells

      ab108310 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as a counter stain and DAPI was used as a nuclear counter stain. 

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

      Lane 1 : THP-1 cell lysate at 10 µg
      Lane 2 : human fetal liver lysate

      Performed under reducing conditions.

      Predicted band size: 110 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Normal liver tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Hepatocellular carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Astrocytoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Normal breast tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Prostatic carcinoma tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Normal tonsil tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Normal stomach tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Normal colon tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 showing positive staining in Lung adenocarcinoma tissue.

    References

    This product has been referenced in:
    • Harada K  et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
    • Nefla M  et al. The pro-inflammatory cytokine 14-3-3e is a ligand of CD13 in cartilage. J Cell Sci 128:3250-62 (2015). IHC . Read more (PubMed: 26208633) »
    See all 5 Publications for this product

    Publishing research using ab108310? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews

    Immunocytochemistry abreview for Anti-CD13 antibody [EPR4058]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Pig Cultured Cells (Vero cells)
    Permeabilization
    No
    Specification
    Vero cells
    Fixative
    Acetone
    Read More

    Mahesh Bhandari

    Verified customer

    Submitted Nov 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (trochlea bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    trochlea bone
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 06 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Jejunum)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Jejunum
    Fixative
    Paraformaldehyde
    Read More

    Dr. Li Li

    Verified customer

    Submitted May 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [EPR4058]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (brain pericytes)
    Specification
    brain pericytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Verena Ledwig

    Verified customer

    Submitted Mar 09 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Normal kidney
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 24 2016

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