Name: Anti-CD13 antibody [EPR4058]
Brand: RabMAb
SKU: ab108310
Rating: 4 (5 reviews)

Product name

Anti-CD13 antibody [EPR4058]
See all CD13 primary antibodies
Description

Rabbit monoclonal [EPR4058] to CD13
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human CD13 aa 400-500. The exact sequence is proprietary.
Positive control
- THP-1 cell lysate, human fetal liver lysate, human kidney tissue
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4058
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- THP1 whole cell lysate (ab7913)

Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IP		1/10 - 1/100.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.

Application notes

Is unsuitable for Flow Cyt.

Function

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Tissue specificity

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Sequence similarities

Belongs to the peptidase M1 family.
Domain

Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
Post-translational
modifications

Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.
Cellular localization

Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
Target information above from: UniProt accession P15144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 290 Human
- Entrez Gene: 16790 Mouse
- Entrez Gene: 81641 Rat
- Omim: 151530 Human
- SwissProt: P15144 Human
- SwissProt: P97449 Mouse
- SwissProt: P15684 Rat
- Unigene: 1239 Human
- Unigene: 4487 Mouse
- Unigene: 11132 Rat
- Unigene: 179371 Rat
see all
Alternative names
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
- Aminopeptidase N antibody
- AMPN_HUMAN antibody
- ANPEP antibody
- AP M antibody
- AP N antibody
- AP-M antibody
- AP-N antibody
- APN antibody
- CD 13 antibody
- CD13 antibody
- CD13 antigen antibody
- gp150 antibody
- hAPN antibody
- LAP 1 antibody
- LAP1 antibody
- Microsomal aminopeptidase antibody
- Myeloid plasma membrane glycoprotein CD13 antibody
- p150 antibody
- PEPN antibody
see all

Western blot - Anti-CD13 antibody [EPR4058] (ab108310)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: CD13 (KO) whole cell lysate (20 µg)
Lane 4: Empty
Lane 5: HeLa whole cell lysate (20 µg)

Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE. Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4058] (ab108310)

Confocal image showing membranous staining in THP-1 cells

ab108310 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as a counter stain and DAPI was used as a nuclear counter stain.
Western blot - Anti-CD13 antibody [EPR4058] (ab108310)

All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

Lane 1 : THP-1 cell lysate at 10 µg
Lane 2 : human fetal liver lysate

Performed under reducing conditions.

Predicted band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Astrocytoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Normal breast tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Prostatic carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Normal tonsil tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Normal stomach tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Normal colon tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

ab108310 showing positive staining in Lung adenocarcinoma tissue.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Harada K et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
Nefla M et al. The pro-inflammatory cytokine 14-3-3e is a ligand of CD13 in cartilage. J Cell Sci 128:3250-62 (2015). IHC . Read more (PubMed: 26208633) »

See all 5 Publications for this product

Publishing research using ab108310? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews

Abcam has not validated the combination of species/application used in this Abreview.

Application

Immunocytochemistry

Sample

Pig Cultured Cells (Vero cells)

Permeabilization

Specification

Vero cells

Fixative

Acetone

Mahesh Bhandari

Verified customer

Submitted Nov 29 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Sheep Tissue sections (trochlea bone)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate

Permeabilization

Specification

trochlea bone

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: RT°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jun 06 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rhesus monkey Tissue sections (Jejunum)

Antigen retrieval step

None

Permeabilization

Specification

Jejunum

Fixative

Paraformaldehyde

Dr. Li Li

Verified customer

Submitted May 31 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Immunocytochemistry/ Immunofluorescence

Sample

Pig Cell (brain pericytes)

Specification

brain pericytes

Blocking step

Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C

Fixative

Paraformaldehyde

Mrs. Verena Ledwig

Verified customer

Submitted Mar 09 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Normal kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Permeabilization

Specification

Normal kidney

Blocking step

BSA as blocking agent for 15 minute(s) · Concentration: 3%

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Feb 24 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-CD13 antibody [EPR4058] (ab108310)

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Immunocytochemistry abreview for Anti-CD13 antibody [EPR4058]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [EPR4058]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]