

Anti-CD13 antibody [EPR4058] (ab108310)
- Datasheet
- References (5)
- Protocols
Overview
-
Product nameAnti-CD13 antibody [EPR4058]
See all CD13 primary antibodies -
DescriptionRabbit monoclonal [EPR4058] to CD13
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human CD13 aa 400-500. The exact sequence is proprietary.
-
Positive control
- THP-1 cell lysate, human fetal liver lysate, human kidney tissue
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR4058
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 110 kDa. | |
IP | 1/10 - 1/100. | |
IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/250 - 1/500. |
Target
-
FunctionBroad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
-
Tissue specificityExpressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
-
Sequence similaritiesBelongs to the peptidase M1 family.
-
DomainAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
-
Post-translational
modificationsSulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
Cellular localizationCell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
- Information by UniProt
-
Database links
- Entrez Gene: 290 Human
- Entrez Gene: 16790 Mouse
- Entrez Gene: 81641 Rat
- Omim: 151530 Human
- SwissProt: P15144 Human
- SwissProt: P97449 Mouse
- SwissProt: P15684 Rat
- Unigene: 1239 Human
see all -
Alternative names
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
Images
-
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty
Lane 3: CD13 (KO) whole cell lysate (20 µg)
Lane 4: Empty
Lane 5: HeLa whole cell lysate (20 µg)Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE. Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
Confocal image showing membranous staining in THP-1 cells
ab108310 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as a counter stain and DAPI was used as a nuclear counter stain.
-
All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution
Lane 1 : THP-1 cell lysate at 10 µg
Lane 2 : human fetal liver lysate
Performed under reducing conditions.
Predicted band size: 110 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310, at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Normal liver tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Hepatocellular carcinoma tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Astrocytoma tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Normal breast tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Prostatic carcinoma tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Normal tonsil tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Normal stomach tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Normal colon tissue.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)ab108310 showing positive staining in Lung adenocarcinoma tissue.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Harada K et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
- Nefla M et al. The pro-inflammatory cytokine 14-3-3e is a ligand of CD13 in cartilage. J Cell Sci 128:3250-62 (2015). IHC . Read more (PubMed: 26208633) »