Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CD13 antibody [EPR4058] - Low endotoxin, Azide free (ab227111)

Overview

  • Product name
    Anti-CD13 antibody [EPR4058] - Low endotoxin, Azide free
    See all CD13 primary antibodies
  • Description
    Rabbit monoclonal [EPR4058] to CD13 - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    aa 400-500.

  • Positive control
    • WB: THP-1, Rat kidney, HAP1, HeLa and Mouse kidney lysates; IHC-P: human kidney, liver, hepatocellular carcinoma, prostatic carcinoma, astrocytoma and breast tissues, mouse and rat kidney tissues; ICC/IF: THP-1 cells
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab227111 is a PBS only buffered version of ab108310, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab108310 for information on protocols, dilutions, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • Tissue specificity
      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • Sequence similarities
      Belongs to the peptidase M1 family.
    • Domain
      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • Post-translational
      modifications
      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • Cellular localization
      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Images

    • This WB data was generated using the same anti-CD13 antibody clone, EPR4058, in a different buffer formulation (cat# ab108310).

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD13 (KO) whole cell lysate (20 µg)
      Lane 4: Empty
      Lane 5: HeLa whole cell lysate (20 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE.  Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310)
    • ab108310 (unpurified), at 1/250, staining CD13 in human kidney tissue by immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 (unpurified) showing positive staining in Hepatocellular carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Astrocytoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Normal breast tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Prostatic carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Normal tonsil tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Normal stomach tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Normal colon tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • ab108310 showing positive staining in Lung adenocarcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108310).

    • This IHC data was generated using the same anti-CD13 antibody clone, EPR4058, in a different buffer formulation (cat# ab108310).

      ab108310 showing positive staining in Normal liver tissue.

    References

    This product has been referenced in:
    • Harada K  et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). Read more (PubMed: 27464948) »
    • Cui SX  et al. 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 tripeptide, inhibits human colonic carcinoma growth by targeting Aminopeptidase N (APN/CD13). Eur J Pharmacol 734:50-9 (2014). Read more (PubMed: 24726845) »
    See all 3 Publications for this product

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