Name: Anti-CD13 antibody [EPR4059]
Brand: RabMAb
SKU: ab108382
Rating: 5 (3 reviews)

Product name

Anti-CD13 antibody [EPR4059]
See all CD13 primary antibodies
Description

Rabbit monoclonal [EPR4059] to CD13
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Rat, Human
Immunogen

Synthetic peptide within Human CD13. The exact sequence is proprietary.
Positive control
- THP-1, U937, and A375 cell lysates, Human kidney tissue
General notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4059
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD13 antibody [EPR4059] - BSA and Azide free (ab196576)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- U937 cytoplasmic extract lysate (ab14904)
Recombinant Protein
- Recombinant human CD13 protein (ab134454)

Our Abpromise guarantee covers the use of ab108382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/500. For unpurified use at 1/50 - 1/100.
WB		1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 110 kDa).
IP		1/10 - 1/100.
IHC-P		1/750. Perform antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt.

Function

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Tissue specificity

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Sequence similarities

Belongs to the peptidase M1 family.
Domain

Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
Post-translational
modifications

Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.
Cellular localization

Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
Target information above from: UniProt accession P15144 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 290 Human
- Entrez Gene: 81641 Rat
- Omim: 151530 Human
- SwissProt: P15144 Human
- SwissProt: P15684 Rat
- Unigene: 1239 Human
- Unigene: 11132 Rat
- Unigene: 179371 Rat
Alternative names
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
- Aminopeptidase N antibody
- AMPN_HUMAN antibody
- ANPEP antibody
- AP M antibody
- AP N antibody
- AP-M antibody
- AP-N antibody
- APN antibody
- CD 13 antibody
- CD13 antibody
- CD13 antigen antibody
- gp150 antibody
- hAPN antibody
- LAP 1 antibody
- LAP1 antibody
- Microsomal aminopeptidase antibody
- Myeloid plasma membrane glycoprotein CD13 antibody
- p150 antibody
- PEPN antibody
see all

Western blot - Anti-CD13 antibody [EPR4059] (ab108382)

All lanes : Anti-CD13 antibody [EPR4059] (ab108382) at 1000 µg

Lane 1 : Rat lung lysates
Lane 2 : A375 (Human malignant melanoma epithelial cell) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 110 kDa
Observed band size: 150 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [EPR4059] (ab108382)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling CD13 with purified ab108382 at 1:500 (0.7 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with Purified ab108382 at 1:750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Immunoprecipitation - Anti-CD13 antibody [EPR4059] (ab108382)

ab108382 (purified) at 1:20 dilution (2ug) immunoprecipitating in THP-1 whole cell lysate. THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+): ab108382 & THP-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108382 in THP-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified ab108382 at 1:750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)This image is courtesy of an anonymous abreview.

Unpurified ab108382 staining CD13 in human kidney tissue sections by Immunohistochemistry (Formaldehyde/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween-20 and blocked for 60 minutes at 25°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody at a dilution of 1/400 for 1 hour at 25°C. An Alexa Flour^® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1200) was used as the secondary antibody.
See Abreview
Western blot - Anti-CD13 antibody [EPR4059] (ab108382)

All lanes : Anti-CD13 antibody [EPR4059] (ab108382) at 1/1000 dilution (unpurified)

Lane 1 : THP-1 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : A375 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Immunohistochemical staining of paraffin-embedded Human kidney tissue using unpurified ab108382 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Prostatic carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Ovarian carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Hepatocellular carcinoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Normal breast tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4059] (ab108382)

Unpurified ab108382 showing positive staining in Normal tonsil tissue.

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Wang YC et al. Notch1 promotes the pericyte-myofibroblast transition in idiopathic pulmonary fibrosis through the PDGFR/ROCK1 signal pathway. Exp Mol Med 51:35 (2019). Read more (PubMed: 30902967) »

See 1 Publication for this product

Publishing research using ab108382? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review

1-3 of 3 Abreviews

Application

Immunohistochemistry free floating

Sample

Rat Tissue sections (Brain cortex)

Specification

Brain cortex

Abcam user community

Verified customer

Submitted Feb 22 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Brain)

Permeabilization

Specification

Brain

Blocking step

Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C

Fixative

Methanol

Victor Bernal

Verified customer

Submitted Jul 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (adult kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: commercial - ZYTOMED's OmniPrep (pH 9)

Permeabilization

Yes - 0.05% tween-20

Specification

adult kidney

Blocking step

commercial - Invitrogen's CAS-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Dec 11 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Location selector

Your location is currently set to:

Custom solutions & partnerships

Recombinant Anti-CD13 antibody [EPR4059] (ab108382)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Immunohistochemistry free floating abreview for Anti-CD13 antibody [EPR4059]

Immunohistochemistry (Frozen sections) abreview for Anti-CD13 antibody [EPR4059]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4059]