Recombinant
RabMAb

Recombinant Anti-CD13 antibody [EPR4059] - BSA and Azide free (ab196576)

Overview

  • Product name

    Anti-CD13 antibody [EPR4059] - BSA and Azide free
    See all CD13 primary antibodies
  • Description

    Rabbit monoclonal [EPR4059] to CD13 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human CD13.

  • Positive control

    • THP-1, U937, and A375 cell lysates, Human kidney tissue
  • General notes

    Ab196576 is the carrier-free version of ab108382. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab196576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 110 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • Tissue specificity

      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • Sequence similarities

      Belongs to the peptidase M1 family.
    • Domain

      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • Post-translational
      modifications

      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Images

    • ab108382 (purified) at 1:20 dilution (2ug) immunoprecipitating in THP-1 whole cell lysate. THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
      Lane 2 (+): ab108382 & THP-1 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108382 in THP-1 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling CD13 with purified ab108382 at 1:500 (0.7 μg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with None. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified ab108382 at 1:750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Immunohistochemical staining of paraffin-embedded Human kidney tissue using unpurified ab108382 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Prostatic carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Normal liver tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Ovarian carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Hepatocellular carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Normal breast tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • Unpurified ab108382 showing positive staining in Normal tonsil tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108382).

    • This IHC data was generated using the same anti-CD13 antibody clone, EPR4059, in a different buffer formulation (cat# ab108382).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with Purified ab108382 at 1:750 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

    • This IHC data was generated using the same anti-CD13 antibody clone, EPR4059, in a different buffer formulation (cat# ab108382).

      Unpurified ab108382 staining CD13 in human kidney tissue sections by Immunohistochemistry (Formaldehyde/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.05% tween-20 and blocked for 60 minutes at 25°C. Antigen retrieval was by heat mediation. Samples were incubated with primary antibody at a dilution of 1/400 for 1 hour at 25°C. An Alexa Flour® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1200) was used as the secondary antibody.

    References

    ab196576 has not yet been referenced specifically in any publications.

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