Recombinant
RabMAb

Recombinant Anti-CD13 antibody [SP182] (ab183358)

Overview

  • Product name

    Anti-CD13 antibody [SP182]
    See all CD13 primary antibodies
  • Description

    Rabbit monoclonal [SP182] to CD13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Pig
  • Immunogen

    Synthetic peptide within Human CD13 aa 250-350 (internal sequence). The exact sequence is proprietary.
    Database link: P15144

  • Positive control

    • IHC-P: Human kidney, tonsil, hepatoma, thymus, lung, liver, placenta, bladder, bladder TCC, cerebellum, and cervix tissue; FC: U937 cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • Tissue specificity

    Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Domain

    Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • Post-translational
    modifications

    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alanyl (membrane) aminopeptidase antibody
    • Alanyl aminopeptidase antibody
    • Aminopeptidase M antibody
    • Aminopeptidase N antibody
    • AMPN_HUMAN antibody
    • ANPEP antibody
    • AP M antibody
    • AP N antibody
    • AP-M antibody
    • AP-N antibody
    • APN antibody
    • CD 13 antibody
    • CD13 antibody
    • CD13 antigen antibody
    • gp150 antibody
    • hAPN antibody
    • LAP 1 antibody
    • LAP1 antibody
    • Microsomal aminopeptidase antibody
    • Myeloid plasma membrane glycoprotein CD13 antibody
    • p150 antibody
    • PEPN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD13 with ab183358 at 1/100 dilution (1.29 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of U937 (Human histiocytic lymphoma cell line) cells labeling CD13 using ab183358 at 1/400 (green). Negative control rabbit IgG (blue).

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human kidney tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human tonsil tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human hepatoma tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human thymus tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human lung tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human liver tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human bladder tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human bladder TCC tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human cerebellum tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human cervix tissue sections labeling CD13 using ab183358 at a 1/100 dilution.

References

ab183358 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Flow Cytometry
Sample
Rabbit Cell (adipose)
Permeabilization
No
Specification
adipose
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 20 2018

Application
Western blot
Sample
Pig Cell lysate - whole cell (transfected chinese hamster ovary cells)
Gel Running Conditions
Reduced Denaturing (8% Laemmli gels)
Loading amount
10 µg
Specification
transfected chinese hamster ovary cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Birke Tews

Verified customer

Submitted Dec 03 2015

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