Product nameAnti-CD13 antibody [WM15]
See all CD13 primary antibodies
DescriptionMouse monoclonal [WM15] to CD13
Tested applicationsSuitable for: Functional Studies, ELISA, Flow Cyt, IHC-Fr, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Human AML cells
EpitopeThe antibody recognizes an extracellular epitope.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from hybridoma culture supernatant. > 95% pure (by SDS-PAGE).
- Anti-CD13 antibody [WM15] (Biotin) (ab130430)
- Anti-CD13 antibody [WM15] (PerCP/Cy5.5®) (ab157316)
- Anti-CD13 antibody [WM15] - BSA and Azide free (ab212426)
- Anti-CD13 antibody [WM15] (Alexa Fluor® 488) (ab239271)
- Anti-CD13 antibody [WM15] (Allophycocyanin) (ab239295)
- Anti-CD13 antibody [WM15] (PE-DyLight™ 594) (ab239330)
- Anti-CD13 antibody [WM15] (FITC) (ab52461)
- Anti-CD13 antibody [WM15], prediluted (Phycoerythrin) (ab69775)
Our Abpromise guarantee covers the use of ab7417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at 10 µg/mg of lysate.|
|ICC/IF||Use a concentration of 10 µg/ml. See Abreview.|
|IHC-P||Use at an assay dependent concentration.|
FunctionBroad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Tissue specificityExpressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Sequence similaritiesBelongs to the peptidase M1 family.
DomainAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.
Cellular localizationCell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
- Information by UniProt
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
ab7417 staining CD13 on the surface of Human peripheral blood leukocytes in Flow Cytometry.
ab7417 staining CD13 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400) for 18 hours at 20°C. An undiluted HRP-conjugated Horse anti-mouse IgG polyclonal was used as the secondary antibody.
Lane 1: Control (no antibody used for IP)
Lane 2: Anti CD13 [WM15], ab7417
Ab7417 immunoprecipitating CD13 in Fibrosarcoma whole cell lysates. For western blotting, primary antibody used was ab7417 at 10µg/mg lysate. Sample was incubated for 2hours at 4°C. A HRP conjugated Rabbit pAb was used as the secondary antibody at 1/100 dilution.
Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).
Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.
This product has been referenced in:
- Xi Y et al. Quantitative profiling of CD13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment. Nanoscale 11:1737-1744 (2019). Read more (PubMed: 30623954) »
- Chen X et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-?B pathway. Cell Death Dis 9:207 (2018). Read more (PubMed: 29434225) »