Overview

  • Product name
    Anti-CD13 antibody [WM15]
    See all CD13 primary antibodies
  • Description
    Mouse monoclonal [WM15] to CD13
  • Host species
    Mouse
  • Tested applications
    Suitable for: Functional Studies, ELISA, Flow Cyt, IHC-Fr, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human AML cells

  • Epitope
    The antibody recognizes an extracellular epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab7417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at 10 µg/mg of lysate.
ICC/IF Use a concentration of 10 µg/ml. See Abreview.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • Tissue specificity
    Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • Sequence similarities
    Belongs to the peptidase M1 family.
  • Domain
    Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • Post-translational
    modifications
    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • Cellular localization
    Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alanyl (membrane) aminopeptidase antibody
    • Alanyl aminopeptidase antibody
    • Aminopeptidase M antibody
    • Aminopeptidase N antibody
    • AMPN_HUMAN antibody
    • ANPEP antibody
    • AP M antibody
    • AP N antibody
    • AP-M antibody
    • AP-N antibody
    • APN antibody
    • CD 13 antibody
    • CD13 antibody
    • CD13 antigen antibody
    • gp150 antibody
    • hAPN antibody
    • LAP 1 antibody
    • LAP1 antibody
    • Microsomal aminopeptidase antibody
    • Myeloid plasma membrane glycoprotein CD13 antibody
    • p150 antibody
    • PEPN antibody
    see all

Images

  • ab7417 staining CD13 on the surface of Human peripheral blood leukocytes in Flow Cytometry.
  • ab7417 staining CD13 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400) for 18 hours at 20°C. An undiluted HRP-conjugated Horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1: Control (no antibody used for IP)
    Lane 2: Anti CD13 [WM15], ab7417

    Ab7417 immunoprecipitating CD13 in Fibrosarcoma whole cell lysates. For western blotting, primary antibody used was ab7417 at 10µg/mg lysate. Sample was incubated for 2hours at 4°C. A HRP conjugated Rabbit pAb was used as the secondary antibody at 1/100 dilution.

    See Abreview

  • Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).

    See Abreview

  • Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chen X  et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-?B pathway. Cell Death Dis 9:207 (2018). Read more (PubMed: 29434225) »
  • Li S  et al. Development and Application of Human Renal Proximal Tubule Epithelial Cells for Assessment of Compound Toxicity. Curr Chem Genom Transl Med 11:19-30 (2017). Read more (PubMed: 28401035) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Miss. Laura Palmer

Verified customer

Submitted Jan 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Miss. Laura Palmer

Verified customer

Submitted Dec 17 2013

Answer

Thank you for your reply.
I cannot recommend this antibody for your purposes. Rat still shows a 77% homology and I am afraid that this could be boarder line and this antibody might cross-react.
It is always very difficult to positively proof that an antibody does not react with a certain species. If we do know this we will mention this on our datasheets in form of a "does not react" note. This indicated that the antibody was experimentally tested not to cross-react.
I attached a list of all our antibodies that react with human but do not react with rat for your information.
Maybe this list will include a suitable antibody for your purposes?
Please feel free to contact me again with any further questions.

Read More

Answer

Thank you for your inquiry.
This antibody is tested and guaranteed to work with human samples. Other species were not experimentally tested by us and we simply do not know i this antibody will cross-react.
Human CD13 show a high homology with CD13 of Gorilla and of non human primates (greater than 85%) and therefore it is very likely that this antibody will cross-react. I attached a overview to this email for your information.
If you are interested in using ab7417 with a species that shows 85% or more homology you might be eligible for our testing discount program.
I hope this information is helpful.
Please do not hesitate to contact me again with any further questions about ab7417 or the testing discount program.

Read More

Question
Answer

DISCOUNT CODE: **********
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab7417 in bovine samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for bovine samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in ab7417.

To our knowledge, ab7417 has not been tested in on bovine samples, but based on sequence homology (there is a 87% level of homology between human and bovine), there is a high chance that this antibody will work for you.

Therefore, I can offer a discount off a future purchase if you buy ab7417 now, test it inbovine samplesand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab7417 inbovine samples. I will then send a discount code. This code must be issued before purchasing ab7417 so please wait for my reply before ordering.

2. Purchase ab7417 either by phone, fax, or online (www.abcam.com).

3. Test it inbovine samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab7417 turns out to be unsuitable forbovine samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Fibrosarcoma HT1080)
Specification
Fibrosarcoma HT1080
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
no gate

Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Fibrosarcoma)
Specification
Fibrosarcoma
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Live cells

Abcam user community

Verified customer

Submitted Apr 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (fibrosarcoma)
Total protein in input
1000 µg
Specification
fibrosarcoma
Immuno-precipitation step
Protein G

Miss. Helen Gillingham

Verified customer

Submitted Apr 09 2008

Answer

Thank you for your enquiry. The best choice for a lysate would be a membrane fraction lysate, not a nuclear lysate, since CD13 is a trans-membrane protein. The antigen is expressed on a variety of tissues. The best choice is ab7913, THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate: https://www.abcam.com/index.html?datasheet=7913 We do not have a membrane fraction lysate for this cell line but this preparation will serve as well. Other tissue lysates that could work as positive controls are suggested by the SwissProt entry (accession number P15144) for CD13: "TISSUE SPECIFICITY: Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients." I hope this helps. Please do not hesitate to contact us if you have any other questions.

Read More

1-10 of 13 Abreviews or Q&A

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