Overview

  • Product name
    Anti-CD13 antibody [WM15]
    See all CD13 primary antibodies
  • Description
    Mouse monoclonal [WM15] to CD13
  • Host species
    Mouse
  • Tested applications
    Suitable for: Functional Studies, ELISA, Flow Cyt, IHC-Fr, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human AML cells

  • Epitope
    The antibody recognizes an extracellular epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab7417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 molecule immunoprecipitates.
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at 10 µg/mg of lysate.
ICC/IF Use a concentration of 10 µg/ml. See Abreview.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • Tissue specificity
    Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • Sequence similarities
    Belongs to the peptidase M1 family.
  • Domain
    Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • Post-translational
    modifications
    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • Cellular localization
    Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alanyl (membrane) aminopeptidase antibody
    • Alanyl aminopeptidase antibody
    • Aminopeptidase M antibody
    • Aminopeptidase N antibody
    • AMPN_HUMAN antibody
    • ANPEP antibody
    • AP M antibody
    • AP N antibody
    • AP-M antibody
    • AP-N antibody
    • APN antibody
    • CD 13 antibody
    • CD13 antibody
    • CD13 antigen antibody
    • gp150 antibody
    • hAPN antibody
    • LAP 1 antibody
    • LAP1 antibody
    • Microsomal aminopeptidase antibody
    • Myeloid plasma membrane glycoprotein CD13 antibody
    • p150 antibody
    • PEPN antibody
    see all

Images

  • ab7417 staining CD13 on the surface of Human peripheral blood leukocytes in Flow Cytometry.
  • ab7417 staining CD13 in Human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% serum for 20 minutes; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/400) for 18 hours at 20°C. An undiluted HRP-conjugated Horse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1: Control (no antibody used for IP)
    Lane 2: Anti CD13 [WM15], ab7417

    Ab7417 immunoprecipitating CD13 in Fibrosarcoma whole cell lysates. For western blotting, primary antibody used was ab7417 at 10µg/mg lysate. Sample was incubated for 2hours at 4°C. A HRP conjugated Rabbit pAb was used as the secondary antibody at 1/100 dilution.

    See Abreview

  • Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).

    See Abreview

  • Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Xi Y  et al. Quantitative profiling of CD13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment. Nanoscale 11:1737-1744 (2019). Read more (PubMed: 30623954) »
  • Chen X  et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-?B pathway. Cell Death Dis 9:207 (2018). Read more (PubMed: 29434225) »
See all 15 Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% Tris-HCl pre-cast gels)
Sample
Human Tissue lysate - whole (Brain)
Specification
Brain
Treatment
Diluted 1:2 in sample buffer treated with Þ-mercaptoethanol
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Miss. Laura Palmer

Verified customer

Submitted Jan 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde

Miss. Laura Palmer

Verified customer

Submitted Dec 17 2013

Application
Flow Cytometry
Sample
Human Cell (Fibrosarcoma HT1080)
Specification
Fibrosarcoma HT1080
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
no gate

Abcam user community

Verified customer

Submitted Apr 07 2009

Application
Flow Cytometry
Sample
Human Cell (Fibrosarcoma)
Specification
Fibrosarcoma
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Live cells

Abcam user community

Verified customer

Submitted Apr 07 2009

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (fibrosarcoma)
Total protein in input
1000 µg
Specification
fibrosarcoma
Immuno-precipitation step
Protein G

Miss. Helen Gillingham

Verified customer

Submitted Apr 09 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080 fibrosarcoma)
Specification
HT1080 fibrosarcoma
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 04 2006

Application
Western blot
Sample
Human Cell lysate - whole cell (HT1080 fibrosarcoma)
Loading amount
10 µg
Specification
HT1080 fibrosarcoma
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10%

Miss. Helen Gillingham

Verified customer

Submitted Jul 20 2006

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