Key features and details
- Mouse monoclonal [WM15] to CD13 (FITC)
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Product nameAnti-CD13 antibody [WM15] (FITC)
See all CD13 primary antibodies
DescriptionMouse monoclonal [WM15] to CD13 (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Human AML cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
PurityProtein A purified
Purification notesPrior to conjugation the antibody was purified by protein A affinity chromatography. Following conjugation, it was purified by size exclusion chromatography. Purity >95% by SDS-PAGE.
- Anti-CD13 antibody [WM15] (Biotin) (ab130430)
- Anti-CD13 antibody [WM15] (PerCP/Cy5.5®) (ab157316)
- Anti-CD13 antibody [WM15] (Alexa Fluor® 488) (ab239271)
- Anti-CD13 antibody [WM15] (APC) (ab239295)
- Anti-CD13 antibody [WM15] (PE/DyLight™ 594) (ab239330)
- Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
- Anti-CD13 antibody [WM15], prediluted (PE) (ab69775)
- Anti-CD13 antibody [WM15] (ab7417)
Our Abpromise guarantee covers the use of ab52461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use 20µl for 106 cells.
or 100 µl of whole blood.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionBroad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
Tissue specificityExpressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
Sequence similaritiesBelongs to the peptidase M1 family.
DomainAmino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form.
Cellular localizationCell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
- Information by UniProt
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
ICC/IF image of ab52461 staining HeLa cells transfected with human CD13 cDNA. The primary antibody was diluted 1/25 and incubated with the sample for 1 hour.
Surface staining of human peripheral blood leukocytes with ab52461.
ab52461 has been referenced in 1 publication.
- Bork S et al. DNA methylation pattern changes upon long-term culture and aging of human mesenchymal stromal cells. Aging Cell 9:54-63 (2010). PubMed: 19895632