Key features and details
- Mouse monoclonal [B-P4] to CD130 (gp130) - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD130 (gp130) antibody [B-P4] - BSA and Azide free
See all CD130 (gp130) primary antibodies
DescriptionMouse monoclonal [B-P4] to CD130 (gp130) - BSA and Azide free
Tested Applications & Species
Application Species Flow CytHuman
Full length protein (Human): Natural soluble gp130 receptor.
ab34325 is a carrier free version of ab27357. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes. This product was changed from ascites to tissue culture supernatant on 4th April 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Concentration information loading...
PurityTissue culture supernatant
Purification notesThis antibody was purified by ion exchange chromatography. It is supplied sterile filtered through 0.22 µm and treated to remove endotoxins.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
Ab27357, staining CD130 (gp130) of Eahy 926 cell line by Flow Cytometry.
This image was generated using the ascites version of the product.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27357).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab34325 has been referenced in 3 publications.
- Lee SY et al. IL-6 trans-signaling system in intra-amniotic inflammation, preterm birth, and preterm premature rupture of the membranes. J Immunol 186:3226-36 (2011). WB, IHC-P ; Human . PubMed: 21282511
- Schmitz J et al. SOCS3 exerts its inhibitory function on interleukin-6 signal transduction through the SHP2 recruitment site of gp130. J Biol Chem 275:12848-56 (2000). PubMed: 10777583
- Wijdenes J et al. Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies. Eur J Immunol 25:3474-81 (1995). PubMed: 8566040