The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
Inhibits CNTF activity and binding to gp130.
Induces gp130 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Belongs to the type I cytokine receptor family. Type 2 subfamily. Contains 5 fibronectin type-III domains. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated.
Chevalier S et al. Interleukin-6 family of cytokines induced activation of different functional sites expressed by gp130 transducing protein. J Biol Chem271:14764-72 (1996).
Read more (PubMed: 8662918) »