Anti-CD130 (gp130) antibody [B-R3] (ab34315)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-CD130 (gp130) antibody [B-R3]
See all CD130 (gp130) primary antibodies -
DescriptionMouse monoclonal [B-R3] to CD130 (gp130)
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Host speciesMouse
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SpecificityRecognizes a 130 kDa protein.
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Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
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Species reactivityReacts with: Human
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Immunogen
Natural soluble gp130 receptor.
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Positive control
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General notes
ab34315 inhibits IL6, IL11, CNTF, LIF and OSM activity. Inhibits OSM and CNTF binding
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituent: PBS
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Concentration information loading... -
Purification notesPurified by Ion exchange chromatography, sterile-filtered through 0.22 µm. Treated to remove endotoxins.
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Primary antibody notesab34315 inhibits IL6, IL11, CNTF, LIF and OSM activity. Inhibits OSM and CNTF binding
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ClonalityMonoclonal
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Clone numberB-R3
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Myelomax63-Ag8.653
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IsotypeIgG2a
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab34315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 103 kDa. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
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Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
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Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Datasheets and documents
References
This product has been referenced in:
- Lamertz L et al. Combined deletion of the fibronectin-type III domains and the stalk region results in ligand-independent, constitutive activation of the Interleukin 6 signal-transducing receptor gp130. Cytokine N/A:N/A (2018). Read more (PubMed: 29789201) »
- Wijdenes J et al. Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies. Eur J Immunol 25:3474-81 (1995). Read more (PubMed: 8566040) »
