Anti-CD130 (gp130) antibody [B-R3] - BSA and Azide free (ab34315)
Key features and details
- Mouse monoclonal [B-R3] to CD130 (gp130) - BSA and Azide free
- Suitable for: Flow Cyt, ELISA, Functional Studies
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CD130 (gp130) antibody [B-R3] - BSA and Azide free
See all CD130 (gp130) primary antibodies -
Description
Mouse monoclonal [B-R3] to CD130 (gp130) - BSA and Azide free -
Host species
Mouse -
Specificity
Recognizes a 130 kDa protein. -
Tested applications
Suitable for: Flow Cyt, ELISA, Functional Studiesmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule corresponding to Human CD130 (gp130).
Database link: P40189 -
General notes
ab34315 inhibits IL6, IL11, CNTF, LIF and OSM activity. Inhibits OSM and CNTF binding
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purification notes
Purified by Ion exchange chromatography, sterile-filtered through 0.22 µm. Treated to remove endotoxins. -
Primary antibody notes
ab34315 inhibits IL6, IL11, CNTF, LIF and OSM activity. Inhibits OSM and CNTF binding -
Clonality
Monoclonal -
Clone number
B-R3 -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
ELISA |
Use at an assay dependent concentration.
|
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Functional Studies |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. |
Functional Studies
Use at an assay dependent concentration. |
Target
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Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab34315 has been referenced in 4 publications.
- Lamertz L et al. Combined deletion of the fibronectin-type III domains and the stalk region results in ligand-independent, constitutive activation of the Interleukin 6 signal-transducing receptor gp130. Cytokine N/A:N/A (2018). PubMed: 29789201
- Lamertz L et al. Soluble gp130 prevents interleukin-6 and interleukin-11 cluster signaling but not intracellular autocrine responses. Sci Signal 11:N/A (2018). PubMed: 30279168
- Ziblat A et al. IL-27 stimulates human NK-cell effector functions and primes NK cells for IL-18 responsiveness. Eur J Immunol 45:192-202 (2015). PubMed: 25308526
- Wijdenes J et al. Interleukin-6 signal transducer gp130 has specific binding sites for different cytokines as determined by antagonistic and agonistic anti-gp130 monoclonal antibodies. Eur J Immunol 25:3474-81 (1995). PubMed: 8566040