Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)
Overview
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Product name
Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free
See all CD130 (gp130) primary antibodies -
Description
Mouse monoclonal [B-S12] to CD130 (gp130) - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to CD130 (gp130).
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Positive control
- Eahy 926 cell line.
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General notes
ab269709 is a carrier free version of ab27359. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes. This product was changed from ascites to tissue culture supernatant on 19th June 2 19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.Induces gp13 activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
B-S12 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab269709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 130 kDa. |
Target
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Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Images
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![Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)](https://www.abcam.com/ps/products/269/ab269709/Images/ab269709-1-38124anticd130gp130antibodybs12flowcytometry.jpg)
Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab269709 has not yet been referenced specifically in any publications.
