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    cd130-gp130-antibody-b-s12-bsa-and-azide-free-ab269709.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)

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Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)

    Key features and details

    • Mouse monoclonal [B-S12] to CD130 (gp130) - BSA and Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

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    Primary
    Product image
    Anti-CD130 (gp130) antibody (ab202850)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Protein
    Recombinant rat CD130 (gp130) protein (Fc Chimera) (ab214595)

    View more associated products

    Overview

    • Product name

      Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free
      See all CD130 (gp130) primary antibodies
    • Description

      Mouse monoclonal [B-S12] to CD130 (gp130) - BSA and Azide free
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Full length native protein (purified) corresponding to CD130 (gp130).

    • Positive control

      • Eahy 926 cell line.
    • General notes

      ab269709 is a carrier free version of ab27359.

      This product was changed from ascites to tissue culture supernatant on 19th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      Induces gp13  activation, Jak 1, Jak 2, Stat 1 and Stat 3 phosphorylation.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.3
      Constituent: PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      B-S12
    • Isotype

      IgG1
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins
      • Immunology
      • Cell Type Markers
      • CD
      • Cytokines
      • Signal Transduction
      • Adapters
      • Transmembrane
      • Cancer
      • Signal transduction
      • Other
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Cardiovascular
      • Heart
      • Apoptosis
      • Cardiovascular
      • Heart
      • Hypertrophy
      • Other

    Associated products

    • Alternative Versions

      • Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab27359)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant rat CD130 (gp130) protein (Fc Chimera) (ab214595)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269709 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
    • Tissue specificity

      Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 2 subfamily.
      Contains 5 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • Domain

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.
    • Post-translational
      modifications

      Phosphorylation of Ser-782 down-regulates cell surface expression.
      Heavily N-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P40189 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3572 Human
      • Omim: 600694 Human
      • SwissProt: P40189 Human
      • Unigene: 532082 Human
      • Unigene: 706627 Human
      • Alternative names

        • CD130 antibody
        • CD130 antigen antibody
        • CDw130 antibody
        • gp130 antibody
        • GP130 RAPS antibody
        • IL 6R beta antibody
        • IL-6 receptor subunit beta antibody
        • IL-6R subunit beta antibody
        • IL-6R-beta antibody
        • IL-6RB antibody
        • IL6 ST antibody
        • IL6RB_HUMAN antibody
        • IL6ST antibody
        • Interleukin 6 receptor subunit beta antibody
        • Interleukin receptor beta chain antibody
        • Interleukin-6 receptor subunit beta antibody
        • Interleukin-6 signal transducer antibody
        • Membrane glycoprotein 130 antibody
        • Membrane glycoprotein gp130 antibody
        • Oncostatin M receptor antibody
        • Oncostatin M receptor alpha subunit antibody
        • Oncostatin-M receptor subunit alpha antibody
        see all

      Images

      • Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)
        Flow Cytometry - Anti-CD130 (gp130) antibody [B-S12] - BSA and Azide free (ab269709)

        A typical staining pattern with ab27359 using Eahy 926 cell line.

        This image was generated using the ascites version of the product.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab27359).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab269709? Please let us know so that we can cite the reference in this datasheet.

      ab269709 has not yet been referenced specifically in any publications.

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