Anti-CD130 (gp130) antibody [B-T2] (ab11462)
Key features and details
- Mouse monoclonal [B-T2] to CD130 (gp130)
- Suitable for: ELISA, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD130 (gp130) antibody [B-T2]
See all CD130 (gp130) primary antibodies -
Description
Mouse monoclonal [B-T2] to CD130 (gp130) -
Host species
Mouse -
Tested applications
Suitable for: ELISA, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Natural soluble gp130 receptor (Human).
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General notes
Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section. Store undiluted. The product can be stored for up to 12 months. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
Purified IgG prepared by ion exchange chromatography. -
Clonality
Monoclonal -
Clone number
B-T2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 0.2µg for 106 cells. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Notes |
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ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. Use 0.2µg for 106 cells. Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
Target
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Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab11462 has been referenced in 1 publication.
- Sommer J et al. Constitutively Active Mutant gp130 Receptor Protein from Inflammatory Hepatocellular Adenoma Is Inhibited by an Anti-gp130 Antibody That Specifically Neutralizes Interleukin 11 Signaling. J Biol Chem 287:13743-51 (2012). PubMed: 22523320