Key features and details
- Rabbit polyclonal to CD130 (gp130) - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD130 (gp130) antibody - C-terminal
See all CD130 (gp130) primary antibodies
DescriptionRabbit polyclonal to CD130 (gp130) - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig, Rhesus monkey
Recombinant fragment within Human CD130 (gp130) (C terminal). The exact sequence is proprietary.
Database link: P40189
- WB: HepG2 whole cell lysate (ab7900). IHC-P: J5 xenograft tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 37,96,104 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
Anti-CD130 (gp130) antibody - C-terminal (ab227058) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
HRP-conjugated anti-rabbit IgG
Predicted band size: 37,96,104 kDa
7.5% SDS-PAGE gel.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD130 (gp130) (green) using ab227058 in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes. Nuclear counterstain: Hoechst 33342.
Paraffin-embedded human J5 xenograft tissue stained for CD130 (gp130) using ab227058 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227058 has not yet been referenced specifically in any publications.