Overview

  • Product name

    Anti-CD130 (gp130) antibody - C-terminal
    See all CD130 (gp130) primary antibodies
  • Description

    Rabbit polyclonal to CD130 (gp130) - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CD130 (gp130) (C terminal). The exact sequence is proprietary.
    Database link: P40189

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900). IHC-P: J5 xenograft tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37,96,104 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
  • Tissue specificity

    Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    Phosphorylation of Ser-782 down-regulates cell surface expression.
    Heavily N-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD130 antibody
    • CD130 antigen antibody
    • CDw130 antibody
    • gp130 antibody
    • GP130 RAPS antibody
    • IL 6R beta antibody
    • IL-6 receptor subunit beta antibody
    • IL-6R subunit beta antibody
    • IL-6R-beta antibody
    • IL-6RB antibody
    • IL6 ST antibody
    • IL6RB_HUMAN antibody
    • IL6ST antibody
    • Interleukin 6 receptor subunit beta antibody
    • Interleukin receptor beta chain antibody
    • Interleukin-6 receptor subunit beta antibody
    • Interleukin-6 signal transducer antibody
    • Membrane glycoprotein 130 antibody
    • Membrane glycoprotein gp130 antibody
    • Oncostatin M receptor antibody
    • Oncostatin M receptor alpha subunit antibody
    • Oncostatin-M receptor subunit alpha antibody
    see all

Images

  • Anti-CD130 (gp130) antibody - C-terminal (ab227058) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 37,96,104 kDa



    7.5% SDS-PAGE gel.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD130 (gp130) (green) using ab227058 in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes. Nuclear counterstain: Hoechst 33342. 

  • Paraffin-embedded human J5 xenograft tissue stained for CD130 (gp130) using ab227058 at 1/500 dilution in immunohistochemical analysis.

References

ab227058 has not yet been referenced specifically in any publications.

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