Product nameAnti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free
See all CD130 (gp130) primary antibodies
DescriptionRabbit monoclonal [EPR21732] to CD130 (gp130) - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CD130 (gp130) aa 300-650. The exact sequence is proprietary.
Database link: P40189
- WB: HAP1 and PC-3 whole cell lysates.
Ab234105 is the carrier-free version of ab217671. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab234105 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab234105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa).
We recommend that the customer optimizes the western blotting conditions.
FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution
Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia cell line) whole cell lysate
Lane 2 : CD130 (gp130) knockout HAP1 whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 104 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 114 seconds
locking and dilution buffer: 5% NFDM/TBST.
ab217671 was shown to specifically react with CD130 (gp130) in wild-type HAP1 cells as the signal was lost in CD130 (gp130) knockout cells. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and ab181602 (Human anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/200000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217671).
ab234105 has not yet been referenced specifically in any publications.