Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free (ab234105)

Overview

  • Product name

    Anti-CD130 (gp130) antibody [EPR21732] - BSA and Azide free
    See all CD130 (gp130) primary antibodies
  • Description

    Rabbit monoclonal [EPR21732] to CD130 (gp130) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD130 (gp130) aa 300-650. The exact sequence is proprietary.
    Database link: P40189

  • Positive control

    • WB: HAP1 and PC-3 whole cell lysates.
  • General notes

    Ab234105 is the carrier-free version of ab217671. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab234105 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa).

We recommend that the customer optimizes the western blotting conditions.

Target

  • Function

    Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
  • Tissue specificity

    Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Post-translational
    modifications

    Phosphorylation of Ser-782 down-regulates cell surface expression.
    Heavily N-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD130 antibody
    • CD130 antigen antibody
    • CDw130 antibody
    • gp130 antibody
    • GP130 RAPS antibody
    • IL 6R beta antibody
    • IL-6 receptor subunit beta antibody
    • IL-6R subunit beta antibody
    • IL-6R-beta antibody
    • IL-6RB antibody
    • IL6 ST antibody
    • IL6RB_HUMAN antibody
    • IL6ST antibody
    • Interleukin 6 receptor subunit beta antibody
    • Interleukin receptor beta chain antibody
    • Interleukin-6 receptor subunit beta antibody
    • Interleukin-6 signal transducer antibody
    • Membrane glycoprotein 130 antibody
    • Membrane glycoprotein gp130 antibody
    • Oncostatin M receptor antibody
    • Oncostatin M receptor alpha subunit antibody
    • Oncostatin-M receptor subunit alpha antibody
    see all

Images

  • All lanes : Anti-CD130 (gp130) antibody [EPR21732] (ab217671) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia cell line) whole cell lysate
    Lane 2 : CD130 (gp130) knockout HAP1 whole cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 104 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?


    Exposure time: 114 seconds


    locking and dilution buffer: 5% NFDM/TBST.

    ab217671 was shown to specifically react with CD130 (gp130) in wild-type HAP1 cells as the signal was lost in CD130 (gp130) knockout cells. Wild-type and CD130 (gp130) knockout samples were subjected to SDS-PAGE. ab217671 and ab181602 (Human anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/200000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217671).

References

ab234105 has not yet been referenced specifically in any publications.

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