Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24557-5] to CD130 (gp130)
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Product nameAnti-CD130 (gp130) antibody [EPR24557-5]
See all CD130 (gp130) primary antibodies
DescriptionRabbit monoclonal [EPR24557-5] to CD130 (gp130)
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Human
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
- Flow Cyt: MG63, Wild-type A549 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionSignal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.
Tissue specificityFound in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast) (Left) / MG-63 (human osteosarcoma fibroblast) (Right) cells labelling CD130 (gp130) with ab283569 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Low expression: Daudi (PMID: 2261637).
Flow cytometric analysis of Wild-type A549 (Human lung carcinoma epithelial cell) (Right) / CD130 (gp130) knockout A549 cells (Left) cells labelling CD130 (gp130) with ab283569 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab283569 (right) or Rabbit IgG (monoclonal) (ab172730) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab283569) or Rabbit IgG (monoclonal) (ab172730) isotype (1x106 in 100µl; with 0.01µg antibody) for 30 min on ice.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1:2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD14 PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab283569 has not yet been referenced specifically in any publications.