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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)

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Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
  • Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
  • Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
  • Immunoprecipitation - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
  • Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24557-50] to CD130 (gp130)
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD130 (gp130) antibody [EPR24557-50]
    See all CD130 (gp130) primary antibodies

  • Description

    Rabbit monoclonal [EPR24557-50] to CD130 (gp130)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type A549, PC-3, Daudi, HepG2, MG-63, NIH/3T3, MEF, C6, Untreated NIH/3T3 and NIH/3T3 treated with PNGase F whole cell lysate; Mouse thymus and lung tissue lysate. IP: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 104 kDa.
IP
1/30.
Notes
WB
1/1000. Predicted molecular weight: 104 kDa.
IP
1/30.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events.

  • Tissue specificity

    Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells.

  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

  • Domain

    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.

  • Post-translational
    modifications

    Phosphorylation of Ser-782 down-regulates cell surface expression.
    Heavily N-glycosylated.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CD130 antibody
    • CD130 antigen antibody
    • CDw130 antibody
    • gp130 antibody
    • GP130 RAPS antibody
    • IL 6R beta antibody
    • IL-6 receptor subunit beta antibody
    • IL-6R subunit beta antibody
    • IL-6R-beta antibody
    • IL-6RB antibody
    • IL6 ST antibody
    • IL6RB_HUMAN antibody
    • IL6ST antibody
    • Interleukin 6 receptor subunit beta antibody
    • Interleukin receptor beta chain antibody
    • Interleukin-6 receptor subunit beta antibody
    • Interleukin-6 signal transducer antibody
    • Membrane glycoprotein 130 antibody
    • Membrane glycoprotein gp130 antibody
    • Oncostatin M receptor antibody
    • Oncostatin M receptor alpha subunit antibody
    • Oncostatin-M receptor subunit alpha antibody
    see all

Images

  • Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    All lanes : Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685) at 1/1000 dilution

    Lane 1 : Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : IL6ST (CD130/gp130) knockout A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    ab283685 was shown to specifically react with IL6ST (CD130/gp130) in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266938 (knockout cell lysate ab257207) was used. Wild-type and IL6ST (CD130/gp130) knockout samples were subjected to SDS-PAGE. ab283685 and ab181602 (Rabbit anti-GAPDH loading control) were incubated for 1 hour at room temperature at 1/1000 dilution and 1/200, 000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure time: 70 seconds

  • Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    All lanes : Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685) at 1/1000 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 4 : MG-63 (human osteosarcoma fibroblast) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 6 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 7 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 104 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    gp130 is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 19915009).

    Low expression: Daudi (PMID: 2261637).

    Samples are non-boiled as boiling may cause protein aggregates. 

    Exposure time: Lanes 1-2 and lane 7: 70 seconds; Lanes 3-6: 26 seconds

  • Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    Western blot - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    All lanes : Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : NIH/3T3 whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 104 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates. 

    Exposure time: Lanes 1-2: 48 seconds; Lanes 3-4: 26 seconds.

     

  • Immunoprecipitation - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    Immunoprecipitation - Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)

    CD130 (gp130) was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug with ab283685 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283685 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10 ug

    Lane 2: ab283685 IP in NIH/3T3 whole cell lysate

    Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab283685 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)
    Anti-CD130 (gp130) antibody [EPR24557-50] (ab283685)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab283685? Please let us know so that we can cite the reference in this datasheet.

ab283685 has not yet been referenced specifically in any publications.

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